Thr158
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Home > Phosphorylation Site Page: > Thr158  -  Tip60 (human)

Site Information
VEVVsPAtPVPSETA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 10800707

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 3 , 5 , 7 , 8 , 9 ) , mass spectrometry (in vitro) ( 6 ) , mutation of modification site ( 1 , 2 , 6 ) , phospho-antibody ( 1 , 2 , 6 ) , western blotting ( 1 , 2 , 6 )
Disease tissue studied:
bone cancer ( 2 ) , lung cancer ( 1 ) , non-small cell lung cancer ( 1 ) , non-small cell lung adenocarcinoma ( 1 ) , melanoma skin cancer ( 1 )
Relevant cell line - cell type - tissue:
A549 (pulmonary) ( 1 ) , BJ (fibroblast) ( 6 ) , HEK293T (epithelial) ( 6 ) , HeLa (cervical) ( 3 , 7 ) , HT-144 (melanocyte) ( 1 ) , Jurkat (T lymphocyte) ( 5 , 8 , 9 ) , U2OS (bone cell) ( 2 )

Upstream Regulation
Regulatory protein:
MAPKAPK5 (human) ( 6 ) , MKK3 (human) ( 2 ) , MKK6 (human) ( 2 ) , P38G (human) ( 6 )
Putative in vivo kinases:
P38A (human) ( 2 , 6 ) , P38D (human) ( 6 ) , VRK1 (human) ( 1 )
Kinases, in vitro:
P38A ( 2 ) , P38A (mouse) ( 6 ) , P38B (human) ( 6 ) , P38D (human) ( 6 ) , P38G (human) ( 6 )
Treatments:
adriamycin ( 1 , 2 ) , ionizing_radiation ( 2 ) , SB203580 ( 2 , 6 ) , siRNA ( 1 , 2 ) , VE-821 ( 1 )

Downstream Regulation
Effects of modification on Tip60:
acetylation ( 1 , 2 , 6 ) , enzymatic activity, induced ( 1 , 2 , 6 ) , intracellular localization ( 1 ) , phosphorylation ( 1 ) , protein stabilization ( 1 ) , ubiquitination ( 1 )
Effects of modification on biological processes:
apoptosis, induced ( 2 ) , carcinogenesis, inhibited ( 6 ) , cell growth, inhibited ( 6 ) , signaling pathway regulation ( 2 ) , transcription, induced ( 2 )

References 

1

García-González R, et al. (2022) The VRK1 chromatin kinase regulates the acetyltransferase activity of Tip60/KAT5 by sequential phosphorylations in response to DNA damage. Biochim Biophys Acta Gene Regul Mech, 194887
36280132   Curated Info

2

Xu Y, et al. (2014) Phosphorylation of Tip60 by p38α regulates p53-mediated PUMA induction and apoptosis in response to DNA damage. Oncotarget 5, 12555-72
25544752   Curated Info

3

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

6

Zheng H, et al. (2013) A Posttranslational Modification Cascade Involving p38, Tip60, and PRAK Mediates Oncogene-Induced Senescence. Mol Cell 50, 699-710
23685072   Curated Info

7

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

8

Possemato A (2010) CST Curation Set: 10722; Year: 2010; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995
Curated Info

9

Possemato A (2009) CST Curation Set: 6369; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pSP
Curated Info

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