Tyr857
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Home > Phosphorylation Site Page: > Tyr857  -  PDGFRB (human)

Site Information
DIMRDSNyISKGSTF   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447509

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 8 ) , [32P] bio-synthetic labeling ( 8 , 12 ) , immunoprecipitation ( 10 ) , mass spectrometry ( 1 , 2 , 3 ) , microscopy-colocalization with upstream kinase ( 9 ) , mutation of modification site ( 4 , 5 , 6 , 10 , 11 , 12 ) , phospho-antibody ( 5 , 6 , 8 , 9 , 11 ) , phosphopeptide mapping ( 8 , 12 ) , western blotting ( 5 , 6 , 9 , 10 )
Disease tissue studied:
liver cancer ( 6 ) , neuroblastoma ( 1 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 5 ) , 3T3 (fibroblast) ( 6 ) , AG1523 (fibroblast) [PDGFRB (human)] ( 12 ) , CHO (fibroblast) [EphB1 (human), transfection] ( 11 ) , epithelial ( 10 ) , F cells (fibroblast) [PDGFRB (human)] ( 9 ) , HepG2 (hepatic) ( 6 ) , PAE (endothelial) ( 4 , 8 ) , SK-N-BE(2) (neural crest) ( 1 )

Upstream Regulation
Regulatory protein:
PDGFRB (human) ( 12 )
Putative in vivo kinases:
PDGFRB (human) ( 8 , 12 )
Kinases, in vitro:
PDGFRB (human) ( 12 )
Putative upstream phosphatases:
ACP1 (human) ( 6 )
Phosphatases, in vitro:
PTPRJ (human) ( 7 )
Treatments:
PDGF ( 4 , 5 , 6 , 8 , 9 , 10 , 11 , 12 ) , vanadate ( 9 )

Downstream Regulation
Effects of modification on PDGFRB:
enzymatic activity, induced ( 4 ) , phosphorylation ( 4 )
Effects of modification on biological processes:
cell growth, altered ( 4 )

References 

1

Palacios-Moreno J, et al. (2015) Neuroblastoma Tyrosine Kinase Signaling Networks Involve FYN and LYN in Endosomes and Lipid Rafts. PLoS Comput Biol 11, e1004130
25884760   Curated Info

2

Rikova K (2012) CST Curation Set: 14270; Year: 2012; Biosample/Treatment: cell line, Tumor pilot study 1mg/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

3

Rikova K (2012) CST Curation Set: 14271; Year: 2012; Biosample/Treatment: cell line, Tumor pilot study 2mg/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

4

Wardega P, Heldin CH, Lennartsson J (2010) Mutation of tyrosine residue 857 in the PDGF beta-receptor affects cell proliferation but not migration. Cell Signal 22, 1363-8
20494825   Curated Info

5

Wu JH, et al. (2005) The platelet-derived growth factor receptor-beta phosphorylates and activates G protein-coupled receptor kinase-2. A mechanism for feedback inhibition. J Biol Chem 280, 31027-35
15994317   Curated Info

6

Chiarugi P, et al. (2002) Insight into the role of low molecular weight phosphotyrosine phosphatase (LMW-PTP) on platelet-derived growth factor receptor (PDGF-r) signaling. LMW-PTP controls PDGF-r kinase activity through TYR-857 dephosphorylation. J Biol Chem 277, 37331-8
12149261   Curated Info

7

Persson C, Engström U, Mowbray SL, Ostman A (2002) Primary sequence determinants responsible for site-selective dephosphorylation of the PDGF beta-receptor by the receptor-like protein tyrosine phosphatase DEP-1. FEBS Lett 517, 27-31
12062403   Curated Info

8

Kovalenko M, et al. (2000) Site-selective dephosphorylation of the platelet-derived growth factor beta-receptor by the receptor-like protein-tyrosine phosphatase DEP-1. J Biol Chem 275, 16219-26
10821867   Curated Info

9

Bernard A, Kazlauskas A (1999) Phosphospecific antibodies reveal temporal regulation of platelet-derived growth factor beta receptor signaling. Exp Cell Res 253, 704-12
10585294   Curated Info

10

Kashishian A, Kazlauskas A, Cooper JA (1992) Phosphorylation sites in the PDGF receptor with different specificities for binding GAP and PI3 kinase in vivo. EMBO J 11, 1373-82
1314164   Curated Info

11

Morrison DK, Kaplan DR, Rhee SG, Williams LT (1990) Platelet-derived growth factor (PDGF)-dependent association of phospholipase C-gamma with the PDGF receptor signaling complex. Mol Cell Biol 10, 2359-66
1691440   Curated Info

12

Kazlauskas A, Cooper JA (1989) Autophosphorylation of the PDGF receptor in the kinase insert region regulates interactions with cell proteins. Cell 58, 1121-33
2550144   Curated Info