Ser861

Site Information
TRYHKRTsSAVWNSP SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 450467

In vivo Characterization
Methods used to characterize site in vivo:	immunoassay ( 1 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 ) , phospho-antibody ( 1 , 10 )
Disease tissue studied:	testicular cancer ( 7 )
Relevant cell line - cell type - tissue:	F9 (testicular) ( 7 ) , HeLa (cervical) ( 4 , 10 ) , Hepa 1-6 (epithelial) ( 8 ) , liver ( 9 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 3 ) , MEF (fibroblast) ( 2 ) , oocyte ( 1 ) , PYS-2 (epithelial) ( 6 ) , spleen ( 5 )

Upstream Regulation
Putative in vivo kinases:	AurC (human) ( 1 )
Kinases, in vitro:	AurB (human) ( 10 )

References
1	Balboula AZ, Schindler K (2014) Selective disruption of aurora C kinase reveals distinct functions from aurora B kinase during meiosis in mouse oocytes. PLoS Genet 10, e1004194 24586209 Curated Info
2	Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51 22942356 Curated Info
3	Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6 21659605 Curated Info
4	Hegemann B, et al. (2011) Systematic phosphorylation analysis of human mitotic protein complexes. Sci Signal 4, rs12 22067460 Curated Info
5	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
6	Zhou J (2010) CST Curation Set: 9675; Year: 2010; Biosample/Treatment: cell line, PY2/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986 Curated Info
7	Zhou J (2010) CST Curation Set: 9671; Year: 2010; Biosample/Treatment: cell line, F9/untreated; Disease: testicular cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986 Curated Info
8	Pan C, Gnad F, Olsen JV, Mann M (2008) Quantitative phosphoproteome analysis of a mouse liver cell line reveals specificity of phosphatase inhibitors. Proteomics 8, 4534-46 18846507 Curated Info
9	Villén J, Beausoleil SA, Gerber SA, Gygi SP (2007) Large-scale phosphorylation analysis of mouse liver. Proc Natl Acad Sci U S A 104, 1488-93 17242355 Curated Info
10	Goto H, et al. (2006) Complex formation of Plk1 and INCENP required for metaphase-anaphase transition. Nat Cell Biol 8, 180-7 16378098 Curated Info