Tyr308
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Home > Phosphorylation Site Page: > Tyr308  -  DDEF1 (mouse)

Site Information
PKEVGGLyVASRANS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450437

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 3 ) , mass spectrometry ( 1 ) , mutation of modification site ( 3 ) , phospho-antibody ( 3 ) , phosphoamino acid analysis ( 3 ) , phosphopeptide mapping ( 3 ) , western blotting ( 3 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 3 ) , liver ( 1 )

Upstream Regulation
Putative in vivo kinases:
Pyk2 (mouse) ( 3 ) , Src iso1 (mouse) ( 3 )
Kinases, in vitro:
Pyk2 (human) ( 2 ) , Pyk2 (mouse) ( 3 )

Downstream Regulation
Effects of modification on DDEF1:
activity, inhibited ( 2 , 3 ) , molecular association, regulation ( 2 , 3 )
Induce interaction with:
Pyk2 (mouse) ( 3 )
Inhibit interaction with:
Other ( 2 )

References 

1

Possemato A (2010) CST Curation Set: 9747; Year: 2010; Biosample/Treatment: tissue, liver/control; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

2

Kruljac-Letunic A, Blaukat A (2005) Assay and functional properties of the tyrosine kinase Pyk2 in regulation of Arf1 through ASAP1 phosphorylation. Methods Enzymol 404, 411-22
16413287   Curated Info

3

Kruljac-Letunic A, et al. (2003) The tyrosine kinase Pyk2 regulates Arf1 activity by phosphorylation and inhibition of the Arf-GTPase-activating protein ASAP1. J Biol Chem 278, 29560-70
12771146   Curated Info

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