Ser64

Site Information
ssWRVVssIEQkTEG SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 10407031

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 2 , 3 , 4 , 5 ) , mass spectrometry (in vitro) ( 1 ) , mutation of modification site ( 1 )
Disease tissue studied:	HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, triple negative ( 2 ) , lung cancer ( 4 ) , non-small cell lung adenocarcinoma ( 4 ) , prostate cancer ( 1 ) , melanoma skin cancer ( 3 )
Relevant cell line - cell type - tissue:	breast ( 2 ) , Jurkat (T lymphocyte) ( 5 ) , LNCaP (prostate cell) ( 1 ) , lung ( 4 ) , WM239A (melanocyte) ( 3 )

Downstream Regulation
Effects of modification on 14-3-3 zeta:	activity, induced ( 1 ) , intracellular localization ( 1 ) , molecular association, regulation ( 1 )
Effects of modification on biological processes:	transcription, induced ( 1 )
Induce interaction with:	AR (human) ( 1 ) , DNA ( 1 )

References
1	Ruff SE, et al. (2021) PIM1 phosphorylation of the androgen receptor and 14-3-3 ζ regulates gene transcription in prostate cancer. Commun Biol 4, 1221 34697370 Curated Info
2	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
3	Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615 25850435 Curated Info
4	Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96 23911959 Curated Info
5	Zhou J (2009) CST Curation Set: 5905; Year: 2009; Biosample/Treatment: cell line, Jurkat/TPA; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)XpSX(K/R)Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) PKC Substrate Antibody Cat#: 2261, PTMScan(R) Phospho-PKC Substrate Motif (K/RXpSXK/R) Immunoaffinity Beads Cat#: 1985 Curated Info