Ser727
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Home > Phosphorylation Site Page: > Ser727  -  STAT3 (rat)

Site Information
NTIDLPMsPRTLDSL   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448191

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 6 ) , mutation of modification site ( 5 , 15 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 9 , 11 , 12 , 13 , 14 )
Disease tissue studied:
adrenal cancer ( 5 ) , pheochromocytoma ( 5 ) , liver cancer ( 12 ) , prostate cancer ( 15 )
Relevant cell line - cell type - tissue:
'brain, forebrain' ( 3 ) , 'kidney, inner medulla' ( 6 ) , 'stem, embryonic' ( 2 ) , astrocyte-'brain, striatum' ( 9 ) , FRTL-5 (thyroid cell) ( 14 ) , granulosa ( 13 ) , H4IIe (hepatic) ( 12 ) , heart ( 4 ) , HIRcB (fibroblast) ( 1 ) , HUVEC (endothelial) ( 8 ) , IEC-6 (epithelial) ( 7 ) , LNCaP (prostate cell) ( 15 ) , lung ( 10 ) , myocardium ( 8 ) , neuron-'brain, cerebral cortex' ( 5 ) , neuron-brain ( 3 ) , NR8383 (macrophage) ( 10 ) , PC-12 (chromaffin) ( 5 ) , Rat1 (fibroblast) [ADRA1A (human)] ( 11 ) , Rat1 (fibroblast) [ADRA1B (human)] ( 11 ) , Rat1 (fibroblast) [ADRA1D (human)] ( 11 )

Upstream Regulation
Regulatory protein:
ADRA1A (human) ( 11 ) , ADRA1B (human) ( 11 ) , ADRA1D (human) ( 11 )
Treatments:
anti-IgG ( 10 ) , BDNF ( 5 ) , C5a ( 10 ) , CNTF ( 3 ) , colforsin ( 14 ) , eflornithine ( 7 ) , H-7 ( 5 ) , H2O2 ( 9 ) , hypoxia/reoxygenation ( 8 ) , IFN-gamma ( 14 ) , IL-22 ( 12 ) , IL-6 ( 11 ) , insulin ( 1 ) , ischemia/reperfusion ( 4 , 8 ) , K252a ( 5 ) , LIF ( 2 ) , NGF ( 5 ) , PD98059 ( 12 ) , pregnancy ( 13 ) , PRL ( 13 ) , putrescine ( 7 ) , racepinefrine ( 11 ) , rottlerin ( 13 ) , seliciclib ( 5 ) , siRNA ( 1 , 5 ) , tempol ( 4 ) , TNF ( 7 ) , TSH ( 14 ) , U0126 ( 5 , 12 ) , wortmannin ( 5 )

Downstream Regulation
Effects of modification on STAT3:
activity, induced ( 1 , 2 , 5 , 15 ) , intracellular localization ( 7 ) , molecular association, regulation ( 1 , 8 )
Effects of modification on biological processes:
apoptosis, altered ( 7 ) , cell differentiation, altered ( 2 ) , transcription, altered ( 1 , 2 , 15 ) , transcription, induced ( 5 )
Induce interaction with:
SP1 (rat) ( 8 )

References 

1

Kwon H, et al. (2009) Caveolin-2 regulation of STAT3 transcriptional activation in response to insulin. Biochim Biophys Acta 1793, 1325-33
19427337   Curated Info

2

Li P, et al. (2008) Germline competent embryonic stem cells derived from rat blastocysts. Cell 135, 1299-310
19109898   Curated Info

3

Nagao M, Sugimori M, Nakafuku M (2007) Cross talk between notch and growth factor/cytokine signaling pathways in neural stem cells. Mol Cell Biol 27, 3982-94
17371842   Curated Info

4

McCormick J, et al. (2006) Free radical scavenging inhibits STAT phosphorylation following in vivo ischemia/reperfusion injury. FASEB J 20, 2115-7
16935931   Curated Info

5

Ng YP, Cheung ZH, Ip NY (2006) STAT3 as a downstream mediator of Trk signaling and functions. J Biol Chem 281, 15636-44
16611639   Curated Info

6

Hoffert JD, et al. (2006) Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites. Proc Natl Acad Sci U S A 103, 7159-64
16641100   Curated Info

7

Bhattacharya S, Ray RM, Johnson LR (2005) STAT3-mediated transcription of Bcl-2, Mcl-1 and c-IAP2 prevents apoptosis in polyamine-depleted cells. Biochem J 392, 335-44
16048438   Curated Info

8

Yang XP, et al. (2005) Signal transducer and activator of transcription 3alpha and specificity protein 1 interact to upregulate intercellular adhesion molecule-1 in ischemic-reperfused myocardium and vascular endothelium. Arterioscler Thromb Vasc Biol 25, 1395-400
15860735   Curated Info

9

Gorina R, Petegnief V, Chamorro A, Planas AM (2005) AG490 prevents cell death after exposure of rat astrocytes to hydrogen peroxide or proinflammatory cytokines: involvement of the Jak2/STAT pathway. J Neurochem 92, 505-18
15659221   Curated Info

10

Gao H, et al. (2004) Stat3 activation in acute lung injury. J Immunol 172, 7703-12
15187153   Curated Info

11

Gonzalez-Cabrera PJ, et al. (2003) Genetic profiling of alpha 1-adrenergic receptor subtypes by oligonucleotide microarrays: coupling to interleukin-6 secretion but differences in STAT3 phosphorylation and gp-130. Mol Pharmacol 63, 1104-16
12695539   Curated Info

12

Lejeune D, et al. (2002) Interleukin-22 (IL-22) activates the JAK/STAT, ERK, JNK, and p38 MAP kinase pathways in a rat hepatoma cell line. Pathways that are shared with and distinct from IL-10. J Biol Chem 277, 33676-82
12087100   Curated Info

13

Peters CA, et al. (2000) Induction of relaxin messenger RNA expression in response to prolactin receptor activation requires protein kinase C delta signaling. Mol Endocrinol 14, 576-90
10770494   Curated Info

14

Park ES, et al. (2000) Thyrotropin induces SOCS-1 (suppressor of cytokine signaling-1) and SOCS-3 in FRTL-5 thyroid cells. Mol Endocrinol 14, 440-8
10707961   Curated Info

15

Udayakumar TS, Stratton MS, Nagle RB, Bowden GT Fibroblast growth factor-1 induced promatrilysin expression through the activation of extracellular-regulated kinases and STAT3. Neoplasia 4, 60-7
11922392   Curated Info