Lys195
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Home > Ubiquitylation Site Page: > Lys195  -  NFkB-p65 (human)

Site Information
APNTAELkICRVNRN   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 10304900

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 ) , mutation of modification site ( 3 ) , phospho-antibody ( 3 ) , western blotting ( 3 )
Relevant cell line - cell type - tissue:
HEK293T (epithelial) ( 3 ) , Jurkat (T lymphocyte) ( 1 ) , MEF (fibroblast) ( 3 )

Downstream Regulation
Effects of modification on NFkB-p65:
activity, inhibited ( 3 ) , protein degradation ( 3 )
Effects of modification on biological processes:
transcription, altered ( 3 )

References 

1

Udeshi ND, et al. (2013) Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Mol Cell Proteomics 12, 825-31
23266961   Curated Info

2

Li H, et al. (2012) Regulation of NF-κB activity by competition between RelA acetylation and ubiquitination. Oncogene 31, 611-23
21706061   Curated Info

3

Fan Y, et al. (2009) Tumor necrosis factor-alpha induces RelA degradation via ubiquitination at lysine 195 to prevent excessive nuclear factor-kappaB activation. J Biol Chem 284, 29290-7
19706600   Curated Info