Ser215
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Home > Phosphorylation Site Page: > Ser215  -  NRF2 (human)

Site Information
VETTMVPsPEAKLTE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 10292704

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 8 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 , 8 ) , mutation of modification site ( 1 , 8 ) , phospho-antibody ( 8 ) , western blotting ( 8 )
Disease tissue studied:
breast cancer ( 1 ) , HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, triple negative ( 3 ) , cervical cancer ( 7 ) , cervical adenocarcinoma ( 7 ) , melanoma skin cancer ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
ERK1 ( 1 ) , JNK1 (human) ( 1 )
Treatments:
SP600125 ( 1 ) , U0126 ( 1 )

Downstream Regulation
Effects of modification on NRF2:
intracellular localization ( 8 ) , molecular association, regulation ( 1 ) , protein stabilization ( 1 )
Effects of modification on biological processes:
transcription, induced ( 8 )
Induce interaction with:
PIN1 (human) ( 1 )

References 

1

Saeidi S, et al. (2022) Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 directly binds and stabilizes Nrf2 in breast cancer. FASEB J 36, e22068
34918396   Curated Info

2

Matzinger M, et al. (2020) AMPK leads to phosphorylation of the transcription factor Nrf2, tuning transactivation of selected target genes. Redox Biol 29, 101393
31805502   Curated Info

3

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

4

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

5

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

6

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

7

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

8

Sun Z, Huang Z, Zhang DD (2009) Phosphorylation of Nrf2 at multiple sites by MAP kinases has a limited contribution in modulating the Nrf2-dependent antioxidant response. PLoS One 4, e6588
19668370   Curated Info