Ser408
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.4
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser408  -  NRF2 (human)

Site Information
GDMVQPLsPSQGQST   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 10292700

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 3 , 6 ) , mass spectrometry ( 3 , 4 , 5 , 6 ) , mutation of modification site ( 1 , 2 , 6 ) , phospho-antibody ( 6 ) , western blotting ( 3 , 6 )
Disease tissue studied:
breast cancer ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
AMPKA1 (human) ( 1 , 3 ) , ERK1 ( 2 ) , JNK1 (human) ( 2 )
Kinases, in vitro:
AMPKA1 (human) ( 3 )
Treatments:
A-769662 ( 1 , 3 ) , compound_C ( 3 ) , MG132 ( 3 ) , SP600125 ( 2 ) , U0126 ( 2 )

Downstream Regulation
Effects of modification on NRF2:
activity, induced ( 3 ) , intracellular localization ( 6 ) , molecular association, regulation ( 1 , 2 , 3 ) , protein degradation ( 1 ) , protein stabilization ( 3 ) , ubiquitination ( 1 )
Effects of modification on biological processes:
signaling pathway regulation ( 3 ) , transcription, induced ( 3 , 6 ) , transcription, inhibited ( 1 )
Induce interaction with:
BTRC (human) ( 3 ) , FBW1B (human) ( 1 ) , PIN1 (human) ( 2 )

References 

1

Petsouki E, Ender S, Sosa Cabrera SN, Heiss EH (2023) AMPK-Mediated Phosphorylation of Nrf2 at S374/S408/S433 Favors Its ╬▓TrCP2-Mediated Degradation in KEAP1-Deficient Cells. Antioxidants (Basel) 12
37627580   Curated Info

2

Saeidi S, et al. (2022) Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 directly binds and stabilizes Nrf2 in breast cancer. FASEB J 36, e22068
34918396   Curated Info

3

Matzinger M, et al. (2020) AMPK leads to phosphorylation of the transcription factor Nrf2, tuning transactivation of selected target genes. Redox Biol 29, 101393
31805502   Curated Info

4

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

5

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

6

Sun Z, Huang Z, Zhang DD (2009) Phosphorylation of Nrf2 at multiple sites by MAP kinases has a limited contribution in modulating the Nrf2-dependent antioxidant response. PLoS One 4, e6588
19668370   Curated Info