Ser2481
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Home > Phosphorylation Site Page: > Ser2481  -  mTOR (mouse)

Site Information
tVPEsIHsFIGDGLV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447498

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 21 ) , immunoassay ( 3 ) , immunoprecipitation ( 18 ) , mass spectrometry ( 7 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 22 , 23 ) , phospho-antibody ( 3 , 4 , 6 , 8 , 16 , 17 , 18 , 19 , 20 , 24 ) , western blotting ( 4 , 6 , 8 , 16 , 17 , 18 , 19 , 20 , 21 , 24 )
Disease tissue studied:
breast cancer ( 8 ) , melanoma skin cancer ( 22 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 7 , 9 , 10 ) , 3T3 (fibroblast) ( 17 , 19 ) , 3T3-L1 (fibroblast) ( 20 ) , brain ( 12 , 13 , 15 ) , breast [PDK1 (mouse), homozygous knockout] ( 8 ) , CHO (fibroblast) [Tau iso8 (human)] ( 24 ) , D3 mES (stem) ( 4 ) , ESC-CMs ('stem, embryonic') ( 4 ) , heart ( 11 ) , hepatocyte-liver ( 6 ) , kidney ( 15 ) , L6 (myoblast) ( 19 ) , liver ( 6 , 15 , 23 ) , lung ( 3 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 14 ) , MEF (fibroblast) ( 16 , 18 , 20 ) , myocyte-heart ( 4 ) , skin [mGluR1 (mouse), transgenic, TG mutant mice] ( 22 ) , T lymphocyte ( 21 ) , testis ( 15 )

Upstream Regulation
Regulatory protein:
ADRB1 (mouse) ( 11 ) , CAMK2G (mouse) ( 6 ) , Cas-L (human) ( 3 ) , P38A (mouse) ( 18 ) , PDK1 (human) ( 8 ) , RICTOR (human) ( 4 ) , Sin1 (mouse) ( 16 ) , TSC1 (mouse) ( 20 )
Putative in vivo kinases:
mTOR (mouse) ( 20 )
Treatments:
2-deoxyglucose ( 20 ) , adriamycin ( 18 ) , amino_acid_starvation ( 19 , 20 ) , amino_acids ( 20 ) , antigenic stimulation ( 21 ) , B7 ( 21 ) , DEN ( 6 ) , IL-12 ( 21 ) , insulin ( 10 , 17 , 19 , 20 ) , LY294002 ( 10 ) , MK-2206 ( 10 ) , rapamycin ( 14 , 17 , 19 , 20 ) , SB203580 ( 18 ) , Torin1 ( 20 ) , troglitazone ( 24 ) , wortmannin ( 19 , 20 )

Downstream Regulation
Effects of modification on mTOR:
enzymatic activity, induced ( 20 )

References 

1

Simula L, et al. (2021) PD-1-induced T cell exhaustion is controlled by a Drp1-dependent mechanism. Mol Oncol
34535949   Curated Info

2

Tooley AS, et al. (2021) The innate immune kinase TBK1 directly increases mTORC2 activity and downstream signaling to Akt. J Biol Chem 297, 100942
34245780   Curated Info

3

Deneka AY, et al. (2021) Nedd9 restrains autophagy to limit growth of early stage non-small cell lung cancer. Cancer Res
34006524   Curated Info

4

Wang JD, et al. (2021) Rictor/mTORC2 involves mitochondrial function in ES cells derived cardiomyocytes via mitochondrial Connexin 43. Acta Pharmacol Sin
33547375   Curated Info

5

Brandt C, et al. (2018) Food Perception Primes Hepatic ER Homeostasis via Melanocortin-Dependent Control of mTOR Activation. Cell 175, 1321-1335.e20
30445039   Curated Info

6

Meng Z, et al. (2017) CAMK2γ antagonizes mTORC1 activation during hepatocarcinogenesis. Oncogene 36, 2446-2456
27819676   Curated Info

7

Minard AY, et al. (2016) mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Rep 17, 29-36
27681418   Curated Info

8

Du J, et al. (2016) PDK1 promotes tumor growth and metastasis in a spontaneous breast cancer model. Oncogene 35, 3314-23
26455327   Curated Info

9

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

10

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

11

Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11
23737553   Curated Info

12

Trinidad JC, et al. (2012) Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse. Mol Cell Proteomics 11, 215-29
22645316   Curated Info

13

Goswami T, et al. (2012) Comparative phosphoproteomic analysis of neonatal and adult murine brain. Proteomics 12, 2185-9
22807455   Curated Info

14

Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6
21659605   Curated Info

15

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

16

Oh WJ, et al. (2010) mTORC2 can associate with ribosomes to promote cotranslational phosphorylation and stability of nascent Akt polypeptide. EMBO J 29, 3939-3951
21045808   Curated Info

17

Soliman GA, Acosta-Jaquez HA, Fingar DC (2010) mTORC1 inhibition via rapamycin promotes triacylglycerol lipolysis and release of free fatty acids in 3T3-L1 adipocytes. Lipids 45, 1089-100
21042876   Curated Info

18

Lai KP, et al. (2010) S6K1 is a multifaceted regulator of Mdm2 that connects nutrient status and DNA damage response. EMBO J 29, 2994-3006
20657550   Curated Info

19

Nascimento EB, et al. (2010) Phosphorylation of PRAS40 on Thr246 by PKB/AKT facilitates efficient phosphorylation of Ser183 by mTORC1. Cell Signal 22, 961-7
20138985   Curated Info

20

Soliman GA, et al. (2010) mTOR Ser-2481 autophosphorylation monitors mTORC-specific catalytic activity and clarifies rapamycin mechanism of action. J Biol Chem 285, 7866-79
20022946   Curated Info

21

Rao RR, Li Q, Odunsi K, Shrikant PA (2010) The mTOR kinase determines effector versus memory CD8+ T cell fate by regulating the expression of transcription factors T-bet and Eomesodermin. Immunity 32, 67-78
20060330   Curated Info

22

Zanivan S, et al. (2008) Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-26
19367708   Curated Info

23

Villén J, Beausoleil SA, Gerber SA, Gygi SP (2007) Large-scale phosphorylation analysis of mouse liver. Proc Natl Acad Sci U S A 104, 1488-93
17242355   Curated Info

24

d'Abramo C, Ricciarelli R, Pronzato MA, Davies P (2006) Troglitazone, a peroxisome proliferator-activated receptor-gamma agonist, decreases tau phosphorylation in CHOtau4R cells. J Neurochem 98, 1068-77
16787414   Curated Info