Thr254
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Home > Phosphorylation Site Page: > Thr254  -  NFkB-p65 (human)

Site Information
QVAIVFRtPPYADPs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450278

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 2 ) , immunoprecipitation ( 1 ) , mutation of modification site ( 1 , 2 ) , phospho-antibody ( 1 , 2 ) , phosphopeptide mapping ( 2 ) , western blotting ( 1 , 2 )
Relevant cell line - cell type - tissue:
HEK293T (epithelial) ( 2 ) , HeLa (cervical) ( 2 ) , macrophage-peritoneum ( 1 ) , MEF (fibroblast) ( 1 , 2 )

Upstream Regulation
Treatments:
TNF ( 2 )

Downstream Regulation
Effects of modification on NFkB-p65:
activity, induced ( 2 ) , intracellular localization ( 2 ) , molecular association, regulation ( 2 ) , protein stabilization ( 2 )
Effects of modification on biological processes:
transcription, altered ( 2 ) , transcription, induced ( 1 )
Induce interaction with:
PIN1 (human) ( 2 )

References 

1

Peng Y, et al. (2016) AGE-RAGE signal generates a specific NF-κB RelA "barcode" that directs collagen I expression. Sci Rep 6, 18822
26729520   Curated Info

2

Ryo A, et al. (2003) Regulation of NF-kappaB signaling by Pin1-dependent prolyl isomerization and ubiquitin-mediated proteolysis of p65/RelA. Mol Cell 12, 1413-26
14690596   Curated Info