Tyr64

Site Information
DTAGQEDyDRLRPLS SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 450272
Associated spectra: 2 CST

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 ) , mutation of modification site ( 4 ) , phospho-antibody ( 4 ) , western blotting ( 4 )
Disease tissue studied:	gastric cancer ( 1 , 3 ) , gastric carcinoma ( 1 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 4 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 4 ) , COS (fibroblast) ( 4 ) , Hs746T (pancreatic) ( 3 ) , MKN-45 (gastric) ( 1 , 2 )

Downstream Regulation
Effects of modification on CDC42 iso1:	activity, induced ( 4 ) , molecular association, regulation ( 4 )
Effects of modification on biological processes:	cell growth, altered ( 4 )
Induce interaction with:	RhoGDI alpha (human) ( 4 )

References
1	Moritz A, et al. (2010) Akt-RSK-S6 Kinase Signaling Networks Activated by Oncogenic Receptor Tyrosine Kinases. Sci Signal 3, ra64 20736484 Curated Info
2	Moritz A (2008) CST Curation Set: 5770; Year: 2008; Biosample/Treatment: cell line, MKN-45/Su11274; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
3	Guo A (2006) CST Curation Set: 1937; Year: 2006; Biosample/Treatment: cell line, Hs746T/-; Disease: pancreatic carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
4	Tu S, Wu WJ, Wang J, Cerione RA (2003) Epidermal growth factor-dependent regulation of Cdc42 is mediated by the Src tyrosine kinase. J Biol Chem 278, 49293-300 14506284 Curated Info