Ser142
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Home > Phosphorylation Site Page: > Ser142  -  CREB (rat)

Site Information
RkILNDLssDAPGVP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448547

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 6 ) , mass spectrometry ( 1 ) , mutation of modification site ( 4 , 5 , 6 ) , phospho-antibody ( 2 , 5 ) , phosphopeptide mapping ( 6 ) , western blotting ( 2 , 6 )
Disease tissue studied:
neuroblastoma ( 4 )
Relevant cell line - cell type - tissue:
'brain, nucleus accumbens' ( 2 ) , CV1 (fibroblast) ( 4 ) , GH3 (epithelial) ( 6 ) , H4IIe (hepatic) ( 5 ) , lung ( 1 ) , SK-N-MC (neural crest) ( 4 ) , spleen ( 1 )

Upstream Regulation
Putative in vivo kinases:
CAMK2A (mouse) ( 2 , 6 )
Kinases, in vitro:
CAMK2A (human) ( 4 ) , CAMK2A (mouse) ( 6 ) , CAMK2A (rat) ( 3 ) , CK2A1 (human) ( 3 )
Treatments:
BDNF ( 3 ) , depolarization ( 3 ) , glutamic acid ( 3 ) , KN-62 ( 3 ) , PD98059 ( 3 ) , PTZ ( 3 )

Downstream Regulation
Effects of modification on CREB:
activity, inhibited ( 4 , 5 ) , molecular association, regulation ( 4 )
Effects of modification on biological processes:
transcription, inhibited ( 4 )
Inhibit interaction with:
CBP (human) ( 4 ) , CBP (rat) ( 3 ) , CREB (rat) ( 4 )

References 

1

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

2

Loweth JA, et al. (2010) Transient overexpression of alpha-Ca2+/calmodulin-dependent protein kinase II in the nucleus accumbens shell enhances behavioral responding to amphetamine. J Neurosci 30, 939-49
20089902   Curated Info

3

Kornhauser JM, et al. (2002) CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events. Neuron 34, 221-33
11970864   Curated Info

4

Wu X, McMurray CT (2001) Calmodulin kinase II attenuation of gene transcription by preventing cAMP response element-binding protein (CREB) dimerization and binding of the CREB-binding protein. J Biol Chem 276, 1735-41
11013247   Curated Info

5

Yeagley D, Agati JM, Quinn PG (1998) A tripartite array of transcription factor binding sites mediates cAMP induction of phosphoenolpyruvate carboxykinase gene transcription and its inhibition by insulin. J Biol Chem 273, 18743-50
9668047   Curated Info

6

Sun P, Enslen H, Myung PS, Maurer RA (1994) Differential activation of CREB by Ca2+/calmodulin-dependent protein kinases type II and type IV involves phosphorylation of a site that negatively regulates activity. Genes Dev 8, 2527-39
7958915   Curated Info