Thr36
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Home > Phosphorylation Site Page: > Thr36  -  4E-BP1 (mouse)

Site Information
PPGDysttPGGtLFs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447497

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 54 ) , immunoprecipitation ( 8 , 39 ) , mass spectrometry ( 3 , 4 , 7 , 10 , 13 , 14 , 15 , 24 , 26 , 28 , 31 , 32 , 33 , 34 , 35 , 36 , 38 , 43 , 44 ) , mutation of modification site ( 50 ) , peptide sequencing ( 29 ) , phospho-antibody ( 1 , 2 , 5 , 8 , 9 , 11 , 17 , 20 , 23 , 25 , 27 , 30 , 37 , 39 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 ) , western blotting ( 1 , 2 , 5 , 8 , 9 , 11 , 17 , 20 , 23 , 25 , 27 , 29 , 30 , 37 , 39 , 46 , 47 , 48 , 49 , 50 , 52 )
Disease tissue studied:
Alzheimer's disease ( 1 ) , anthrax infection ( 26 ) , bone cancer ( 50 ) , osteosarcoma ( 50 ) , melanoma skin cancer ( 38 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 4 , 7 , 14 ) , 'brain, hippocampus' [FKBP12 (mouse), homozygous knockout] ( 39 ) , 'brain, hippocampus, dentate gyrus' [FKBP12 (mouse), homozygous knockout] ( 39 ) , 'neuron, cortical' ( 17 ) , 'neuron, hippocampal' ( 17 ) , 'stem, embryonic' ( 32 ) , 293 (epithelial) ( 47 ) , 293T (epithelial) ( 47 ) , 3T3 (fibroblast) ( 47 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 54 ) , 3T3-L1 (fibroblast) ( 30 ) , BMMC (mast) ( 27 ) , C2C12 (myoblast) ( 46 , 51 ) , C2C12 (myoblast) [LIN28A (mouse), transfection] ( 20 ) , heart ( 35 , 36 , 43 ) , HeLa (cervical) ( 11 , 25 , 47 ) , hepatocyte-liver ( 2 ) , hepatocyte-liver [TSC1 (mouse), homozygous knockout] ( 23 ) , intestine ( 5 ) , JB (epithelial) ( 52 ) , liver ( 2 , 3 , 13 , 33 , 34 , 44 ) , macrophage-bone marrow ( 1 , 28 ) , macrophage-bone marrow [DUSP1 (mouse), homozygous knockout] ( 28 ) , macrophage-peritoneum ( 15 ) , macrophage-peritoneum [MPRIP (mouse), homozygous knockout] ( 15 ) , MEF (fibroblast) ( 11 , 24 , 25 , 30 , 47 , 48 , 49 ) , MEF (fibroblast) [Sin1 (mouse), homozygous knockout] ( 37 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 24 ) , mpkCCD (renal) ( 31 ) , NMuMG (epithelial) ( 8 ) , RAW 264.7 (macrophage) ( 10 ) , skin [mGluR1 (mouse), transgenic, TG mutant mice] ( 38 ) , spleen ( 26 ) , thymocyte ( 29 )

Upstream Regulation
Regulatory protein:
Akt2 (mouse) ( 29 ) , CAMK2G (mouse) ( 2 ) , Ezrin (mouse) ( 50 ) , FKBP12 (mouse) ( 39 ) , FOXO1A (mouse) ( 46 ) , GADD34 (human) ( 45 ) , LIN28A (human) ( 20 ) , LIN28A (mouse) ( 20 ) , LIN28B (mouse) ( 20 ) , LKB1 (mouse) ( 42 ) , LST8 (mouse) ( 48 ) , Musashi-1 (mouse) ( 5 ) , PTGER3 (mouse) ( 27 ) , Raptor (mouse) ( 27 ) , RICTOR (mouse) ( 27 ) , Sin1 (mouse) ( 37 , 49 ) , TSC1 (mouse) ( 23 ) , TSC2 (mouse) ( 47 )
Putative in vivo kinases:
MSK1 (human) ( 52 ) , mTOR (mouse) ( 54 ) , P38A (human) ( 52 )
Kinases, in vitro:
mTOR (rat) ( 55 ) , P38A (human) ( 52 )
Treatments:
AMDE-1 ( 11 ) , AS252424 ( 27 ) , butaprost ( 27 ) , BX795 ( 37 ) , cell_detachment ( 47 ) , culturing_of_cells ( 17 ) , DEN ( 2 ) , exercise ( 42 ) , FGF1 ( 41 ) , FGF2 ( 41 ) , glucose ( 40 , 53 ) , H-89 ( 52 ) , IC87114 ( 27 ) , INK-128 ( 8 ) , insulin ( 8 , 14 , 20 , 23 , 37 , 40 , 49 , 53 , 54 ) , KU-0063794 ( 24 ) , let-7 miRNA ( 20 ) , LY294002 ( 14 , 50 ) , PD169316 ( 52 ) , PD98059 ( 52 ) , PGE2 ( 27 ) , PIK90 ( 37 ) , PP242 ( 29 , 37 ) , PTX ( 27 ) , rapamycin ( 20 , 23 , 27 , 29 , 30 , 37 , 50 , 51 , 52 , 54 ) , SB202190 ( 52 ) , serum ( 20 , 49 ) , serum_starvation ( 20 , 49 ) , siRNA ( 50 ) , sulprostone ( 27 ) , tamoxifen ( 46 ) , Torin1 ( 8 , 27 , 30 ) , U0126 ( 50 , 52 ) , UV ( 52 ) , wortmannin ( 1 , 27 , 52 )

Downstream Regulation
Effects of modification on 4E-BP1:
molecular association, regulation ( 51 )
Induce interaction with:
EIF4E (mouse) ( 51 )
Inhibit interaction with:
EIF4E (mouse) ( 46 )

References 

1

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2

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3

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8

Im YK, et al. (2015) The ShcA adaptor activates AKT signaling to potentiate breast tumor angiogenesis by stimulating VEGF mRNA translation in a 4E-BP-dependent manner. Oncogene 34, 1729-35
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9

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10

Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44
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11

Li M, et al. (2015) AMDE-1 is a dual function chemical for autophagy activation and inhibition. PLoS One 10, e0122083
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12

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13

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14

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15

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16

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17

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18

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19

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20

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21

Peterson TR, et al. (2011) mTOR complex 1 regulates lipin 1 localization to control the SREBP pathway. Cell 146, 408-20
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22

Ryu JM, Han HJ (2011) L-threonine regulates G1/S phase transition of mouse embryonic stem cells via PI3K/Akt, MAPKs, and mTORC pathways. J Biol Chem 286, 23667-78
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23

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24

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25

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26

Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927
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27

Kuehn HS, et al. (2011) Prostaglandin E2 activates and utilizes mTORC2 as a central signaling locus for the regulation of mast cell chemotaxis and mediator release. J Biol Chem 286, 391-402
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28

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29

Hsieh AC, et al. (2010) Genetic dissection of the oncogenic mTOR pathway reveals druggable addiction to translational control via 4EBP-eIF4E. Cancer Cell 17, 249-61
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30

Soliman GA, et al. (2010) mTOR Ser-2481 autophosphorylation monitors mTORC-specific catalytic activity and clarifies rapamycin mechanism of action. J Biol Chem 285, 7866-79
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31

Rinschen MM, et al. (2010) Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells. Proc Natl Acad Sci U S A 107, 3882-7
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32

Tucker M (2009) CST Curation Set: 8601; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

33

Zhou J (2009) CST Curation Set: 7446; Year: 2009; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
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34

Zhou J (2009) CST Curation Set: 7447; Year: 2009; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
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35

Zhou J (2009) CST Curation Set: 7444; Year: 2009; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
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36

Zhou J (2009) CST Curation Set: 7445; Year: 2009; Biosample/Treatment: tissue, heart/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
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37

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38

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39

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40

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41

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42

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43

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Curated Info

44

Zhou J (2008) CST Curation Set: 3810; Year: 2008; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995
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45

Minami K, et al. (2007) Suppression of viral replication by stress-inducible GADD34 protein via the mammalian serine/threonine protein kinase mTOR pathway. J Virol 81, 11106-15
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46

Southgate RJ, et al. (2007) FOXO1 regulates the expression of 4E-BP1 and inhibits mTOR signaling in mammalian skeletal muscle. J Biol Chem 282, 21176-86
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47

Gan B, Yoo Y, Guan JL (2006) Association of focal adhesion kinase with tuberous sclerosis complex 2 in the regulation of s6 kinase activation and cell growth. J Biol Chem 281, 37321-9
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48

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49

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50

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51

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52

Liu G, et al. (2002) Phosphorylation of 4E-BP1 is mediated by the p38/MSK1 pathway in response to UVB irradiation. J Biol Chem 277, 8810-6
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53

Patel J, Wang X, Proud CG (2001) Glucose exerts a permissive effect on the regulation of the initiation factor 4E binding protein 4E-BP1. Biochem J 358, 497-503
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54

Mothe-Satney I, et al. (2000) Mammalian target of rapamycin-dependent phosphorylation of PHAS-I in four (S/T)P sites detected by phospho-specific antibodies. J Biol Chem 275, 33836-43
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55

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