Lys222

Site Information
AFERLEVkkETLLAM SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 10041712

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 )
Relevant cell line - cell type - tissue:	HEp-2 (squamous) ( 1 ) , NCI-H1299 (pulmonary) [VCP (human), transfection, VCP siRNA construct was used to transfect cells] ( 2 )

Upstream Regulation
Regulatory protein:	VCP (human) ( 2 )
Treatments:	siRNA ( 2 )

References
1	Akimov V, et al. (2018) UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites. Nat Struct Mol Biol 25, 631-640 29967540 Curated Info
2	Vasilescu J, et al. (2007) The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells. J Proteome Res 6, 298-305 17203973 Curated Info