Ser16
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Home > Phosphorylation Site Page: > Ser16  -  PLB (dog)

Site Information
RSAIRRAstIEMPQQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448114

In vivo Characterization
Methods used to characterize site in vivo:
phospho-antibody ( 1 , 3 , 5 ) , western blotting ( 3 )
Relevant cell line - cell type - tissue:
heart ( 3 , 5 ) , myocyte-heart ( 1 )

Upstream Regulation
Kinases, in vitro:
CAMK2A (human) ( 4 ) , PKACA (dog) ( 4 , 6 ) , PKACA (human) ( 2 )
Treatments:
heart failure ( 3 )

Downstream Regulation
Effects of modification on PLB:
molecular association, regulation ( 2 )
Inhibit interaction with:
SERCA2 (human) ( 2 )

References 

1

El-Armouche A, et al. (2007) Decreased phosphorylation levels of cardiac myosin-binding protein-C in human and experimental heart failure. J Mol Cell Cardiol 43, 223-9
17560599   Curated Info

2

Chen Z, Akin BL, Jones LR (2007) Mechanism of reversal of phospholamban inhibition of the cardiac Ca2+-ATPase by protein kinase A and by anti-phospholamban monoclonal antibody 2D12. J Biol Chem 282, 20968-76
17548345   Curated Info

3

Mishra S, Sabbah HN, Jain JC, Gupta RC (2003) Reduced Ca2+-calmodulin-dependent protein kinase activity and expression in LV myocardium of dogs with heart failure. Am J Physiol Heart Circ Physiol 284, H876-83
12424092   Curated Info

4

Mayer EJ, Huckle W, Johnson RG, McKenna E (2000) Characterization and quantitation of phospholamban and its phosphorylation state using antibodies. Biochem Biophys Res Commun 267, 40-8
10623571   Curated Info

5

Mayer EJ, Savage GM, Johnson RG (1998) A quantitative immunoassay for the measurement of phospholamban levels and phosphorylation states. Measurement of phospholamban levels in transgenic mouse hearts. Ann N Y Acad Sci 853, 284-7
10603960   Curated Info

6

Simmerman HK, et al. (1986) Sequence analysis of phospholamban. Identification of phosphorylation sites and two major structural domains. J Biol Chem 261, 13333-41
3759968   Curated Info