(        10        20        30        40        50        60        70        80        90       100       110       120       130       140       150       160       170       180       190       200       210       220       230       240       250       260       270       280       290       300       310       320       330       340       350       360       370       380       390       400       410       420       430       440       450       460       470       480       490       500       510       520       530       540       550       560       570       580       590       600       610       620       630       640       650       660       670       680       690       700       710       720       730       740       750       760       770       780       790       800       810       820       830       840       850       860       870       880       890       900       910       920       930       940       950       960       970       980       990      1000      1010      1020      1030      1040      1050      1060      1070      1080      1090      1100      1110      1120      1130      1140      1150      1160      1170      1180      1190      1200      1210      1220      1230      1240      1250      1260      1270      1280      1290      1300      1310      1320      1330      1340      1350      1360      1370      1380      1390      1400      1410      1420      1430      1440      1450      1460      1470      1480      1490      1500      1510      1520      1530      1540      1550      1560      1570      1580      1590      1600      1610      1620      1630      1640      1650      1660      1670      1680      1690      1700      1710      1720      1730      1740      1750      1760      1770      1780      1790      1800      1810      1820      1830      1840      1850      1860      1870      1880      1890      1900      1910      1920      1930      1940      1950      1960      1970  )
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MYH3 human	MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTYCFVVDSkEEYAKGKIKSSQDGKVTVETEDNRTLVVKPEDVyAMNPPKFDRIEDMAMLTHLNEPAVLYNLKDRyTSWMIYTYSGLFCVTVNPYKWLPVYNPEVVEGYRGKKRQEAPPHIFSISDNAyQFMLtDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKD--SKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGkFIRIHFGTtGKLAsADIETyLLEkSRVTFQLKAERSYHIFYQILSNKKPELIELLLITTNPYDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEEKSGLYKLtGAVMHyGNMKFKQKQREEQAEPDGtEVADKTAYLMGLNSSDLLKALCFPRVKVGNEyVtkGQTVDQVHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYkKEGIEWTFIDFGMDLAACIELIEK-------PMGIFSILEEECMFPKATDTSFKNKLyDQHLGKSNNFQKPKVVKGRAEAHFSLIHYAGTVDYSVSGWLEKNKDPLNETVVGLyQKSSNRLLAHLYATFATADADSGK--KKVAKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGFPNRILyGDFKQRyRVLNAsAIPEGQFIDsKKACEKLLASIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDDRLAKLITRTQAVCRGFLMRVEFQKMVQRRESIFCIQyNIRSFMNVKHWPWMkLFFKIKPLLkSAETEKEMAtMKEEFQKtKDELAKSEAKRkELEEKLVTLVQEKNDLQLQVQAESENLLDAEERCDQLIKAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECsELkkDIDDLELtLAKVEKEKHATENKVKNLTEELsGLDETIAKLTREKKALQEAHQQALDDLQAEEDKVNSLNKTKSKLEQQVEDLESSLEQEKKLRVDLERNKRKLEGDLKLAQEsILDLENDKQQLDERLKKKDFEYCQLQSKVEDEQTLGLQFQKKIKELQARIEELEEEIEAERAtRAKtEKQRsDyARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEAMVAALRKKHADsVAELGEQIDNLQRVKQKLEKEKSEFKLEIDDLSSSMESVSKSK----ANLEKICRTLEDQLSEARGKNEEIQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRSKQAFTQQTEELKRQLEEENKAKNALAHALQsSRHDCDLLREQYEEEQEGKAELQRALSKANsEVAQWRtKyEtDAIQRtEELEEAkkkLAQRLQDSEEQVEAVNAKCASLEKtKQRLQGEVEDLMVDVERANSLAAALDKKQRNFDKVLAEWKTKCEESQAELEASLKESRsLstELFKLKNAyEEALDQLETVKRENKNLEQEIADLTEQIAENGKTIHELEKSRKQIELEKADIQLALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQSALDAEVRSRNEAIRLKKKMEGDLNEIEIQLSHANRQAAETLKHLRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVERRANLLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLEtDLMQLQsEVEDASRDARNAEEKAKKAItDAAMMAEELkKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLETRIRELEFELEGEQkKNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQVKVkSYkRQAEEA-----------------DEQANAHLTKFRKAQHELEEAEERADIAEsQVNKLRAKTRDFTSSRMVVHESEE
MYH3 mouse	MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTyCFVVDSKEEYVKGkIKssQDGkVTVETEDSRTLVVkPEDVYAMNPPKFDKIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYNPEVVDGYRGKKRQEAPPHIFSISDNAYQFMLTDREnQSILITGESGAGkTVNTKRVIQyFATIAATGDLAKKKD--SKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGkFIRIHFGTTGKLASADIETYLLEkSRVTFQLkAERSYHIFYQILSNKKPELIELLLITTNPYDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEEKSGLYKLTGAVMHYGNMkFKQkQREEQAEPDGTEVADKTAYLMGLNSSDLLKALCFPRVkVGNEyVtKGQTVDQVHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCINFTNEkLQQFFNHHMFVLEQEEYkKEGIEWTFIDFGMDLAACIELIEK-------PMGIFSILEEECMFPKATDTSFKNKLyDQHLGKSNNFQkPKVVKGKAEAHFSLVHYAGTVDYSVSGWLEKNkDPLNETVVGLYQKSSNRLLAHLYATFATTDADGGK--KKVAKKkGsSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGFPNRILYGDFKQRYRVLNASAIPEGQFIDSk K740-ac K740-ub kACEKLLASIDIDHTQYKFGHTkVFFkAGLLGTLEEMRDERLAKLITRTQAVCRGFLMRVEFQKMMQRRESIFCIQYNIRAFMNVKHWPWMKLFFKIKPLLkSAETEkEMATMKEEFQkTKDELAKSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLLDAEERCDQLIKAKFQLEAKIKEVTERAEDEEEINAELTAkKRkLEDECsELk K952-ac K952-ub kDIDDLELTLAkVEkEKHATENKVKNLTEELAGLDETIAKLTREKkALQEAHQQTLDDLQAEEDKVNSLSKLKSKLEQQVDDLESSLEQEKKLRVDLERNKRkLEGDLKLAQESILDLENDKQQLDERLKKKDFEySQLQSKVEDEQTLSLQLQKKIKELQARIEELEEEIEAERATRAKTEKQRSDYARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEATVATLRKKHADSAAELAEQIDNLQRVKQKLEKEKSEFKLEIDDLSSSVESVSKSK----ANLEkICRTLEDQLSEARGKNEEMQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRsKQAFTQQIEELk K1317-ac K1317-ub RQLEEENKAkNALAHALQsSRHDCDLLREQYEEEQEGKAELQRALskANsEVAQWRtk K1375-ac K1375-ub yEtDAIQRTEELEEAk K1391-ac K1391-ub K1391-sc kKLAQRLQDSEEQVEAVNAKCASLEktKQRLQGEVEDLMVDVERANSLAAALDKKQRNFDkVLAEWKTKCEESQAELEAALKESRsLstELFkLKNAYEEALDQLETVKRENKNLEQEIADLTEQIAENGKSIHELEKSRKQMELEKADIQMALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQGALDAEVRsRNEAIRLKKKMEGDLNEIEIQLSHANRQAAETIKHLRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVERRANLLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLETDLTQLQSEVEDACRDARNAEEKAKkAITDAAMMAEELKKEQDTSAHLERMKkNLEQTVkDLQHRLDEAEQLALkGGkkQIQKLETRIRELEFELEGEQKRNTESVKGLRKYERRVKELTyQSEEDRKNVLRLQDLVDKLQVKVKSYKRQAEEA-----------------DEQANAHLTKFRKAQHELEEAEERADIAEsQVNkLRAKTRDFTSSRMVVHESEE
MYH3 rat	MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTYCFVVDSKEEYAKGKIKSSQDGKVTVETEDNRTLVVKPEDVYAMNPPKFDKIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYTPEVVDGYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKD--SKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGttGKLASADIETYLLEkSRVTFQLkAERSYHIFYQILSNKKPELIELLLITTNPYDYPFISQGEILVASIDDREELLATDSAIDILGFTPEEKSGLYKLTGAVMHYGNMkFKQKQREEQAEPDGtEVADKTAYLMGLNSSDLLKALCFPRVkVGNEyVtKGQTVDQVHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYkKEGIEWTFIDFGMDLAACIELIEK-------PMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQkPKVVKGKAEAHFSLIHYAGTVDYSVSGWLEKNkDPLNEtVVGLYQKSSNRLLAHLYATFATTDADGGK--KKVAKKKGSsFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGFPNRILYGDFKQRYRVLNAsAIPEGQFIDskKACEKLLASIDIDHTQYkFGHTkVFFKAGLLGTLEEMRDERLAKLITRTQAVCRGFLMRVEFQKMMQRRESIFCIQYNIRAFMNVkHWPWMKLFFkIKPLLkSAETEkEMATMkEEFQKTkDELAkSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLLDAEERCDQLIKAKFQLEAKIkEVTERAEDEEEINAELTAkKRkLEDECsELkkDIDDLELtLAkVEkEKHATENkVKNLTEELAGLDETIAKLTREKKALQEAHQQtLDDLQAEEDKVNSLSKLKSKLEQQVDDLESSLEQEKKLRVDLERNKRkLEGDLKLAQEsILDLENDKQQLDERLKKKDFEYSQLQSKVEDEQTLSLQLQKKIkELQARIEELEEEIEAERATRAKTEKQRSDYARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEATVATLRKKHADSAAELAEQIDNLQRVKQKLEkEKSEFKLEIDDLSSSVESVSKSK----ANLEKICRTLEDQLSEARGKNEETQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRSKQAFtQQIEELkRQLEEENKAKNALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANsEVAQWRtkyEtDAIQRtEELEEAkKKLAQRLQDSEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLAAALDKKQRNFDkVLAEWKTKCEESQAELEAALKESRsLstELFKLKNAYEEALDQLETVKRENKNLEQEIADLTEQIAENGKSIHELEKSRKQMELEKADIQMALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEkDEEIEQLkRNYQRTVETMQGALDAEVRSRNEAIRLKKKMEGDLNEIEIQLSHANRQAAETIKHLRSVQGQLKDtQLHLDDALRGQEDLKEQLAIVERRANLLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLETDLTQLQSEVEDASRDARNAEEKAKKAItDAAMMAEELkkEQDTSAHLERMKKNLEQTVkDLQHRLDEAEQLALkGGkKQIQKLETRIRELEFELEGEQKRNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQVKVKSYKRQAEEA-----------------DEQANVHLTKFRKAQHELEEAEERADIAEsQVNkLRAKTRDFTSSRMVVHESEE
MYH3 chicken	MATDADMAIFGEAAPYLRKSEKERIEAQNKPFDAkSSVFVVHAKESYVKSTIQSKESGKVTVKTEGGETLTVKEDQIFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYkWLPVYNPEVVLAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAASGDKKKEEQPAGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPELIEMLLITTNPYDYQYVSQGEITVPSINDQEELMATDSAIDILGFTPDEKTAIYKLTGAVMHYGNLKFKQKQREEQAEPGGTEVADKAAYLMGLNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNSVGALAKSVFEKMFLWMVVRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEK-------PMGIFSILEEECMFPKATDTSFkNKLYDQHLGKSNNFQKPKPGKGKAEAHFSLVHYAGTVDYNITGWLEKNKDPLNETVVGLYQKSSLKTLALLFASVGGAEAESGAGGKKGGKKKGSSFQTVSALFRENLNKLMSNLRSTHPHFVRCLIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPIRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDVDhTQYKFGHTKVFFKAGLLGLLEEMRDEKLAQLITRTQARCRGFLMRVEFKKMMERRESIFCIQYNVRAFMNVKHWPWMKLFFKIKPLLKSAESEKEMANMKEEFEKTKEELAKSEAKRKELEEKMVSLLQEKNDLQLQVQAEADGLADAEERCDQLIKTKIQLEAKIKELTERAEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAALDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKMTQESTMDLENDKQQLDEKLKKKDFEISQIQSKIEDEQALGMQLQKKIKELQARIEELEEEIEAERTSRAKAEKHRADLSRELEEISERLEEAGGATAAQIDMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSTADVGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMESVSKAK----ANLEKMCRSLEDQLSEIKTKEEEQQRTINDISAQKARLQTESGEYSRQVEEKDALISQLSRGKQAFTQQIEELKRHLEEEIKAKKCPAHALQSARHDCDLLREQYEEEQEAKGELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDVERSNAACAALDKKQKNFDKILSEWKQKYEETQAELEASQKESRSLSTELFKMKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKAIHELEKVKKQIEQEKSELQTALEEAEASLEHEEGKILRVQLELNQVKSDIDRKIAEKDEEIDQLKRNHLRVVDSMQSTLDAEIRSRNEALRLKKKMEGDLNEIEIQLSHANRQAAEAQKNLRNTQGVLKDTQIHLDDALRSQEDLKEQVAMVERRANLLQAEIEELRAALEQTERSRKVAEQELLDASERVQLLHTQNTSLINTKKKLESDISQIQSEMEDTIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLDQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVDAEQKRSAEAVKGVRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQMKVKSYKRQAEEA-----------------EELSNVNLSKFRKIQHELEEAEERADIAESQVNKLRAKSREIGKK----AESEE
	*::*::*::**:***:*************:*****:::***::**:*:*::*:*:::*****:**:::**:**::::::*****:*:******:***:*********:**::***********************:****.:***********************************************************:**::*:::  :**:****************************************:***********************************:*********:**********:::*****:*:**:*:***:*************:**:::************:*************:*******:********:*******:****************:**:::*:**:***:**:*****:*********:***:*************::**************************************:*****************       *****************************************::**:*******:********:::**********************:::**:*:*::::::*::*:  **::**************************:********:*************:***********************:****:******:******************:****:***:********************:******::**:*******:**********:**::***********:*:************************:*****:*****:***:***************:*:*:*************:::*:***********:*:********:*********:*************************************************:::*********:***********:************:*.*:*:******:***:*********:****:*********::***::***********:*******::*:***:****:*::*:**********************::***:**:*:*::*****:*********:*::**::********:*:*************::*:********:*:::*******************:*:*****:*::*****:*    *****:**:*******:::*:**.**:::::::**:*****:**:***:***::::*****:*******:*****:****:***:::******:****************:*:********************************************:**:*****:*************:******:****:*:::*******:****:*:***:*:**:******::************:******:**:***:*******:***:********:**:******::**:*:**:::*.*******:*****:****:****:****:************:*****::*:*::**::****:******:**************************:.*:**::**:*****:*******:*******:*:***********:*****:******:**:*******::**************:******:*:.*:***:**::::***************************************:*****************************:*:****:*:::***:::*:***:********************************:************                 :*::*::*:****:************************:*::::::::::****
Consensus	MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTYCFVVDSKEEYAKGKIKSSQDGKVTVETEDNRTLVVKPEDVYAMNPPKFDKIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYNPEVVDGYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKD--SKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPELIELLLITTNPYDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEEKSGLYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKTAYLMGLNSSDLLKALCFPRVKVGNEYVTKGQTVDQVHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEK-------PMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQKPKVVKGKAEAHFSLIHYAGTVDYSVSGWLEKNKDPLNETVVGLYQKSSNRLLAHLYATFATADADSGK--KKVAKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGFPNRILYGDFKQRYRVLNASAIPEGQFIDSKKACEKLLASIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDERLAKLITRTQAVCRGFLMRVEFQKMMQRRESIFCIQYNIRAFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLLDAEERCDQLIKAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEELAGLDETIAKLTREKKALQEAHQQTLDDLQAEEDKVNSLNKLKSKLEQQVDDLESSLEQEKKLRVDLERNKRKLEGDLKLAQESILDLENDKQQLDERLKKKDFEYSQLQSKVEDEQTLGLQLQKKIKELQARIEELEEEIEAERATRAKTEKQRSDYARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEATVAALRKKHADSAAELGEQIDNLQRVKQKLEKEKSEFKLEIDDLSSSMESVSKSK----ANLEKICRTLEDQLSEARGKNEEIQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRSKQAFTQQIEELKRQLEEENKAKNALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDSEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLAAALDKKQRNFDKVLAEWKTKCEESQAELEASLKESRSLSTELFKLKNAYEEALDQLETVKRENKNLEQEIADLTEQIAENGKSIHELEKSRKQIELEKADIQLALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQSALDAEVRSRNEAIRLKKKMEGDLNEIEIQLSHANRQAAETIKHLRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVERRANLLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLETDLMQLQSEVEDASRDARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLETRIRELEFELEGEQKRNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQVKVKSYKRQAEEA-----------------DEQANAHLTKFRKAQHELEEAEERADIAESQVNKLRAKTRDFTSSRMVVHESEE