(        10        20        30        40        50        60        70        80        90       100       110       120       130       140       150       160       170       180       190       200       210       220       230       240       250       260       270       280       290       300       310       320       330       340       350       360       370       380       390       400       410       420       430       440       450       460       470       480       490       500       510       520       530       540       550       560       570       580       590       600       610       620       630       640       650       660       670       680       690       700       710       720       730       740       750       760       770       780       790       800       810       820       830       840       850       860       870       880       890       900       910       920       930       940       950         )
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Trap150 human	MsKTNKSKSGSRSSRSRSASRSRsRsFsKsRsRsRsLsRSRKRRLssRSRsRsysPAHNRErNHPr R66-m1 R66-m2 VyQNr R71-m1 R71-m2 DFr R74-m1 R74-m2 GHNrGYrRPYYFrGRNr R91-m1 R91-m2 GFYPWGQYNr R101-m1 R101-m2 GGYGNyr R108-m1 R108-m2 SNWQNyrQAysPRRGRSRSRSPKRRsPsPRSRSHsRNSDKsssDRsRRSsSSRSssNHSRVESSKRKSAkEKKsssKDsRPsQAAGDNQGDEAk K202-m2 K202-ub K202-sm EQTFsGGtsQDtKAsEssk K221-ac K221-ub PWPDA-TyGtGsAsRAsAVsELsPRERsPALksPLQsVVVRRRsPRPsPVPKPsPPLSSTSQMGSTLPSGAGYQsGTHQGQFDHGSGSLsPsKKsPVGKsPPstGstyGssQKEEsAAsGGAAytKRyLEEQKTENGKDKEQKQtNtDKEKIKEKGsFsDtGLGDGk K387-ac K387-sm MKsDsFAPKtDsEkPFRGsQsPKRyKLRDDFEkKMADFHkEEMDDQDKDKAKGRkEsEFDDEPk K451-ub K451-sm FMSk K455-ac K455-ub VI-GANkNQEEEksGk K470-ac K470-ub K470-sm WEGLVyAPPGkEkQRktEELEEEsFPERskKEDRG--KRsEGGHrGFVPEk K519-ac K519-ub K519-pro NFRVTAykAVQEKSssPPPRKTSESRDKLGAkGDFPTGk K558-ac K558-ub SsFsITREAQVNVRMDsFDEDLARPSGLLAQERKLCRDLVHsNKKEQEFRSIFQHIQSAQsQRsPsELFAQHIVTIVHHVkEHHFGSSGMTLHERFTKyLKRGtEQEAAKNKKsPEIHRRIDIsPstFRKHGLAHDEMksPREPGykAEGk K709-ac K709-pro yk K711-m1 K711-sm DDPVDLRLDIERRKKHKERDLKRGKsREsVDsRDssHsRERsAEkTEkTHKGSKKQKKHRRARDRSRSSSSSSQsSHSYKAEEytEEtEErEEsttGFDk K811-ac K811-ub SRLGTKDFVGPsErGGGrArGtFQFr R837-m1 R837-m2 ArGr R841-m1 R841-m2 GWGr R845-m1 R845-m2 GNYSGNNNNNSNNDFQKRNREEEWDPEytPksKkyyLHDDrEGEGsDKWVSrGrGRGAFPrGrGrFMFRKsstsPKWAHDkFsGEEGEIEDDEsGtENREEKDNIQPttE
Trap150 mouse	MSKTNKSKSGSRSSRSRSASRSRSRSFSKSRSRSRSVSRSRKRRLSsRsRsRsysPAHNRERNHPrVYQNrDFRGHNRGYRRPYYFRGRNrGFYPWGQYNrGGYGNYrSNWQNYRQAYsPRRGRsRsRsPKRRsPsPRSRSHSRNSDKSSSDRSRRSSSSRSSSNHsRVEssKRKSTKEKKsssKDsRPsQAAGDNQGDEAKEQtFsGGTsQDIKGSESSKPWPDATTYGAGsASRA-SVsDLsPRERsPALKsPLQsVVVRRRsPRPsPVPKPsPPLSNASQMGSSMSGGAGYQSGAHQGQFDHGSGsLsPSKKsPVGKsPPAtGsAyGssQKEEsAAsGGAAYSKRYLEEQktENGkDKEQKQTNADkEKLKEKGGFsDA---DVKMKSDPFAPKtDSEkPFRGsQsPKRYKLRDDFEkKMADFHKEELDEHDKDKSKGRKEPEFDDEPKFMSKVIAGASKNQEEEKsGkWESLH---TGkEKQRKAEEMEDEPFTERSRKEERGGSKRSEsGHRGFVPEkNFRVTAYKAVQEKsSsPPPRKTSESRDKLGsKGDFSSGkssFsITREAQVNVRMDsFDEDLARPSGLLAQERKLCRDLVHsNKKEQEFRSIFQHIQSAQSQRsPsELFAQHIVTIVHHVKEHHFGSSGMTLHERFTKYLKRGNEQEAAKNKKsPEIHRRIDIsPstFRKHGLtHEELKsPREPGYKAEGKYKDDPVDLRLDIERRKKHKERDLKRGKsREsVDsRDssHsRERsTEKTEKTHKGSKKQKKHRRARDRSRSSSSSSQSSHSYKAEEYPEEAEEREEsTsGFDKSRLGTKDFVGPNERG-GrArGTFQFRARGRGWGrGNYSGNNNNNSNNDFQKRsREEEWDPEytPKSKKYYLHDDREGEGSDKWMGRGRGRGAFPRGrGrFMFRKsstsPKWAHDKFsGEEGEIEDDEsGtENREEKDsLQPsAE
Trap150 rat	MSKTNKSKSGSRSSRSRSASRSRSRSFSKSRSRSRSVSRSRKRRLSSRSRsRsYsPAHNRERNHPRVYQNRDFRGHNRGYRRPYYFRGRNRGFYPWGQYNRGGYGNYRSNWQNYRQAYsPRRGRSRSRSPKRRsPsPRSRSHSRNSDKSSSDRSRRSSSSRSSSNHSRVESSKRKSTKEKKSSSKDSRPSQAAGDNQGDEAKEQTFSGGTSQDIKGSESSkPWPDATTYGTGSASRA-SVsDLsPRERsPALKsPLQSVVVRRRsPRPSPVPKPSPPLSNASQMGSSMSGGAGYQSGAHQGPFDHGSGSLSPSKKsPVGKsPPATGSAYGSSQKEESAASGGAAYTKRYLEEQKTENGKDKEQKQTNTDKEKLKEkGGFsDA---DVKMKSDPFAPKTDTEkPFRGsQsPKRYKLRDDFEkKMADFHKEEMDEQDkDKCKGRKEPEFDDEPKFISkVIAGASkNQEEEKSGkWESLH---AGkEKQRKAEELEEEPFTERSRKEERGGSKRSESGHRGFVPEkNFRVTAYkAVQEKSSsPPPRKTSESRDKLGNKGDFSSGkSSFSITREAQVNVRMDsFDEDLARPSGLLAQERKLCRDLVHSNKKEQEFRSIFQHIQSAQSQRSPSELFAQHIVTIVHHVKEHHFGSSGMTLHERFTkYLKRGNEQEAAKTKKsPEIHRRIDIsPstFRKHGLTHEELKsPREPGYkAEGKYKDDPVDLRLDIERRKKHKERDLKRGKSRESVDSRDSSHSRERSTEKTEKTHKGSKKQKKHRRARDRSRSSSSSSQSSHSYKAEEYPEEAEDREESTTGFDKSRLGTKDFVGPNERG-GRARGTFQFRARGRGWGRGNYSGNNNNNSNNDFQKRsREEEWDPEYtPKSKKYYLHDDREGEGSDKWMSRGRGRGAFPRGRGRFMFRKSSTSPKWAHDKFsGEEGEIEDDEsGTENREEKDSLQPSAE
Trap150 cow	MSKTNKSKSGSRSSRSRSASRSRSRSFSKSRSRSRSVSRSRKRRLSSRSRSRSYSPAHNRERNHPRVYQNRDFRGHNRGYRRPYYFRGRNRGFYPWGQYNRGGYGNYRSNWQNYRQAYsPRRGRSRSRSPKRRSPSPRSRSHSRNSDKSSSDRSRRSSSSRSSSNHSRVESSKRKSAKEKKSSSKDSRPSQAAGDNQGDEAKEQPFSGGTSQDPKASESSKPWPDATTYSAGSASRASGVSELsPRERsPALKSPLQSVVVRRRSPRPSPVPKPSPPLSSTSQMGSTLQSGAGYQGGTHQGQFDHGSGSLSPSKKSPVGKSPPATGSTYGSSQKEEAAAPGGAAYTKRYLDEQKTENGKDKEQKQTNTDKEKMKEKGSFSDTGLGDTKMKSDSFAPKTDTEKPFRGSQSPKRYKLRDDFEKKMADFHKEEMDDQDKDKAKGRKESEFDDEPKFMSKVIAGASKNQEEEKSGKWEGLVYAPPGKEKQRKTEELEEESFSERSKKEERGGSKRSESGHRGFVPEKNFRVTAYKAVQEKSSSPPPRKTSESREKLGAKGDFSTGKSSFSITREAQVNVRMDsFDEDLARPSGLLAQERKLCRDLVHSNKKEQEFRSIFQHIQSAQSQRSPSELFAQHIVTIVHHVKEHHFGSSGMTLHERFTKYLKRGSEQEAAKNKKsPEIHRRIDISPSTFRKHGLAHDEMKSPREPGYKAEGKYKDDPVDLRLDIERRKKHKERDLKRGKSRESVDSRDSSHSRERSAEKTEKTHKGSKKQKKHRRARDRSRSSSSSSQSSHSYKAEEYTEETEEREESTTGFDKSRLGTKDFVGPSERG-GRARGTFQFRARGRGWGRGNYSGNNNNSSNNDFQKRSREEEWDPEYTPKSKKYYLHDDREGEGSEKWVSRGRGRGAFPRGRGRFMFRKSSTSPKWAHDKFSGEEGEIEDDESGTENREEKDNLQPTTE
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Consensus	MSKTNKSKSGSRSSRSRSASRSRSRSFSKSRSRSRSVSRSRKRRLSSRSRSRSYSPAHNRERNHPRVYQNRDFRGHNRGYRRPYYFRGRNRGFYPWGQYNRGGYGNYRSNWQNYRQAYSPRRGRSRSRSPKRRSPSPRSRSHSRNSDKSSSDRSRRSSSSRSSSNHSRVESSKRKSAKEKKSSSKDSRPSQAAGDNQGDEAKEQTFSGGTSQDIKASESSKPWPDATTYGAGSASRASSVSDLSPRERSPALKSPLQSVVVRRRSPRPSPVPKPSPPLSNASQMGSSLSGGAGYQSGAHQGQFDHGSGSLSPSKKSPVGKSPPATGSAYGSSQKEESAASGGAAYTKRYLEEQKTENGKDKEQKQTNTDKEKLKEKGGFSDAGLGDVKMKSDPFAPKTDSEKPFRGSQSPKRYKLRDDFEKKMADFHKEEMDDQDKDKAKGRKEPEFDDEPKFMSKVIAGASKNQEEEKSGKWEGLHYAPPGKEKQRKAEELEEEPFTERSRKEERGGSKRSESGHRGFVPEKNFRVTAYKAVQEKSSSPPPRKTSESRDKLGAKGDFSSGKSSFSITREAQVNVRMDSFDEDLARPSGLLAQERKLCRDLVHSNKKEQEFRSIFQHIQSAQSQRSPSELFAQHIVTIVHHVKEHHFGSSGMTLHERFTKYLKRGNEQEAAKNKKSPEIHRRIDISPSTFRKHGLAHDELKSPREPGYKAEGKYKDDPVDLRLDIERRKKHKERDLKRGKSRESVDSRDSSHSRERSAEKTEKTHKGSKKQKKHRRARDRSRSSSSSSQSSHSYKAEEYPEEAEEREESTTGFDKSRLGTKDFVGPNERG-GRARGTFQFRARGRGWGRGNYSGNNNNNSNNDFQKRSREEEWDPEYTPKSKKYYLHDDREGEGSDKWMSRGRGRGAFPRGRGRFMFRKSSTSPKWAHDKFSGEEGEIEDDESGTENREEKDNLQPSAE