(        10        20        30        40        50        60        70        80        90       100       110       120       130       140       150       160       170       180       190       200       210       220       230       240       250       260       270       280       290       300       310       320       330       340       350       360       370       380       390       400       410       420       430       440       450       460       470       480       490       500       510       520       530       540       550       560       570       580       590       600       610       620       630       640       650       660       670       680       690       700       710       720       730       740       750       760       770       780       790       800       810       820       830       840       850       860       870       880       890       900       910       920       930       940       950       960       970       980       990      1000      1010      1020      1030      1040      1050      1060      1070      1080      1090      1100      1110      1120      1130      1140      1150      1160      1170      1180      1190      1200      1210      1220      1230      1240      1250      1260     )
	|         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |
RAPGEF1 human	-----------------MDTDSQRSHLSsFTMKLMDKFHsPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGyPLPLDLEQQAVEFMsTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKtIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVkELVRLTIEKQGRPsPTsPVKPssPASKPDGPAELPLTDREVEILNKTTGMSQsTELLPDAtDEEVAPPKPPLPGIRVVDNsPPPALPPKKRQsAPsPTRVAVVAPMsRAtsGssLPVGINRQDFDVDCyAQRRLsGGsHSyGGEsPRLsPCssIGKLsKsDEQLSsLDRDsGQCSRNTSCETLDHYDPDyEFLQQDLSNADQIPQQTAWNLSPLPESLGESGsPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRsAAsQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPyAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDySEPQPSMFyQtPQNEHIyQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQL--------------------------------------------------------------------------------------------------------------------------------------------------------------------------EPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKsPDALESAQSEEEVDELSLIDHNEIMSRLTLkQEGDDGPDVRGGSGDILLVHATETDRKDLVLyCEAFLTTyRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDkVDQKKLLRCAtsSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAkEQNEE-KSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQtsEGLAEYCTLIDSSssFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNItRRKtDREEKt
RAPGEF1 iso2 human	-----------------MDTDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVN---------------------------------------NLSWLEEkEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQL--------------------------------------------------------------------------------------------------------------------------------------------------------------------------EPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEE-KSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT
RAPGEF1 mouse	MSSGLGLRRsPEMSGKIEKADSQRSHLSsFTMKLMDKFHsPKIKRTPSKKGKPAEVS-KIPEKPVsKEARDRFLPEGYPIPLDLEQQAVEFMSTSAVASRSQRQKNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTsPVKPSSPASKPDGQPELPLTDREMEILNKtTsVsPsAELLPDSTSEEVAPPKPPLPGIRVVDNsP-PALPPKKRQsAPsPtRVAVVAPMSRATsGssLPVGINRQDFDVECYTQRRLsGGSRsCGGEsPRLsPCSSTGKLSRsDEQLSSLDRDsGQCsRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQE-----GQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPyPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDySEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQL--------------------------------------------------------------------------------------------------------------------------------------------------------------------------EPPSGKDGHPRDPSV-SSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRtFIsPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEE-KSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNItRRKtDREEKT
RAPGEF1 iso2 mouse	MSSGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVS-KIPEKPVS--------------------------------------KNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSP-PALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQE-----GQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGASSASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSESFCGSDSTQELAPPPALPPKQRQLQASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGsLPVPSYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTVDGPLSSSQDSSFHGNPVRLPSETSFTDSSEKASSEEAGGDEYVSLYSSGQTSEELAPCRGEPPSGKDGHPRDPSV-SSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEE-KSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT
RAPGEF1 rat	---------------------------------------------------------------------------------------------------------------------------FKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRITIEKQGRPSPTSPVKPSSPASKPDGQPELPLTEREMEILNKT-SVSPSTELLPDSTSEEVAPPKPPLPGIRVVDNSP-PALPPKKRQSAPSPTRVAVVAPMSRAVsGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRsDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQTPPQAACNLSPLPESMGESGPPFVGYSFQLPLGSCLQQD-----GQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEDDLQNP--AQPVSYPPFAAILPFQQGASSTPVEFVGDFSVPESAGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSDSFCGSDSTQELAPPPALPPKQRQL-ASYAASSFSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLNRHGSLPVPS----------------------------------------------------------------------------------------------------------------------EPPSGKDGHPRDLSV-SSASGKDSRENGERSPKSLDALESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLyCEAFLTTYRTFISPEELIKKLQCRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEELKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQVHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKtDREEKT
	...................::::::::::::::::::::::::::::::::::::::.:::::::.... ......... .........................::.:::::::::::::::**********************************:***************************************************************:*************************::*****:**:******:::*:*:*****:*:********************.**************************:****************:**:********:*:************:****:*******************************************:*:*:*:********:****:**:*.:******:::*::.....******************:***:*:******************:.***:::::::*:*:****:****:*:**:::******::**::**:*************************************:*********:********:**:*:**:**************** ........... .......................................                                                                                                                      ***:********:*:.*:::*****:::**:***:*:**********************:***********************************************************:*****************************************************:**************************:*:***************************************************** *************************************************************************:*******************************************************************************:**::*********************************************
Consensus	-----------------EDADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVS-KIPEKPVN--------------------------------------KNLCWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQPELPLTDREMEILNKTTSVSPSTELLPDSTSEEVAPPKPPLPGIRVVDNSP-PALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQD-----GQQTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEEDLQNPAPIQPVPYPPFAAILPFQQGASSAPVEFVGDFSVPESAGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLMEVYGFSDSFCGSDSTQELAPPPALPPKQRQL--------------------------------------------------------------------------------------------------------------------------------------------------------------------------EPPSGKDGHPRDPSV-SSASGKDSRENGERSPKSLDALESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEE-KSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT