(        10        20        30        40        50        60        70        80        90       100       110       120       130       140       150       160       170       180       190       200       210       220       230       240       250       260       270       280       290       300       310       320       330       340       350       360       370       380       390       400       410       420       430       440       450       460       470       480       490       500       510       520       530       540       550       560       570       580       590       600       )
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PLK1 human	MsAAVtAGk K9-ac K9-ub LARAPADPGkAGVPGVAAPGAPAAAPPAkEIPEVLVDPRsRRRYVRGRFLGkGGFAkCFEISDADTkEVFAGkIVPkSLLLkPHQREKMsMEIsIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRsLLELHkRRkALTEPEARYYLRQIVLGCQYLHRNRVIHRDLkLGNLFLNEDLEVkIGDFGLATkVEYDGERKk K209-me K209-m1 K209-ub tLCGtPNyIAPEVLskKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKkNEYSIPkHINPVAASLIQkMLQTDPTARPTINELLNDEFFTSGyIPARLPITCLTIPPRFsIAPssLDPsNRkPLtVLNkGLENPLPERPR---EkEEPVVREtGEVVDCHLsDMLQQLHsVNAsk K388-m1 K388-ub PSERGLVRQEEAEDPACIPIFWVSk K413-me K413-ub WVDYSDkYGLGyQLCDNSVGVLFNDSTRLILyNDGDsLQYIERDGtEsyLtVsSHPNSLMk K474-m2 K474-ub KITLLkYFRNYMSEHLLk K492-m2 K492-ub K492-sm AGANItPREGDELARLPYLRTWFRTRSAIILHLsNGsVQINFFQDHtkLILCPLMAAVTYIDEKRDFRTYRLsLLEEYGCCk K574-m1 K574-ub ELAsRLRYARTMVDkLLSSRSAsNRLKAS-
PLK1 mouse	MNAAAKAGKLARAPADLGKGGVPGDAVPGAPVAAPLAKEIPEVLVDPRSRRQYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQKEKMSMEISIHRSLAHQHVVGFHDFFEDSDFVFVVLELCRRRsLLELHKRRKALTEPEARYYLRQIVLGCQyLHRNQVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYEGERKKtLCGtPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTIHELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSSRKPLKVLNKGVENPLPDRPR---EKEEPVVRETNEAIECHLSDLLQQLTSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILyNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLNYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGTVQINFFQDHTKLILCPLMAAVTYINEKRDFQTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS-
PLK1 rat	MNAAAKAGKLARAPADLGKGGVPGDAAPGAPGAAPLAKEIPEVLMDPRSRRQYVRGRFLGKGGFAKCFEISDSDTKEVFPGKIVPKSLLLKPHQKEKMSMEISIHRSLAHQHVVGFHGFFEDSDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNQVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYEGERKKtLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTIHELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGVENPLPDRPR---EKEEPVVRETNEAIECHLSDLLQQLTSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLNYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGTVQINFFQDHTKLILCPLMAAVTYINEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSACNRLKAS-
PLK1 frog	-MAQVAGKKLTVAP--------EAAKPPGIPGSssAVKEIPEILVDPRTRRRYLRGRFLGKGGFAKCYEITDLESREVFAGKIVPKTMLLKPHQKDKMTMEIAIQRSLDHRHVVGFHGFFEDNDFVYVVLELCRRRSLLELHKRRKAVTEPEARYYLKQTISGCQYLHSNRVIHRDLKLGNLFLNDEMEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLGKKGHSFEVDIWSIGCIMYTLLVGKPPFETSCLKETYMRIKKNEYSIPKHINPVAAALIQKMLRSDPTSRPTIDDLLNDEFFTSGYIPSRLPTTCLTVPPRFSIAPSTIDQsLRKPLTAINKGQDsPLVEKQVAPAKEEEMQQPEFTEPADCYLSEMLQQLTCLNAVKPSERALIRQEEAEDPASIPIFWISKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLIMYNDGDSLQYIERNNTESYLNVRSYPTTLTKKITLLKYFRNYMSEHLLKAGANTTPREGDELARLPFLRTWFRTRSAIILHLSNGTVQINFFQDHTKIILCPLMAAVSYIDEKREFRTYKLSLIQEFGCCKELASRLRYARTMVEKLQSSKSAVAHVKASA
	:.*:::::**::**::.::::::::::**:*::::::*****:*:***:**:*:*************:**:*::::***:******::******::**:***:*:***:*:******:****:***:********************:*********:*:::******:*:**************:::***************:*******************:*********:**************************:******************:******::***:****.:*************:***:****:*********::*:*.****:::***.::**:::::   ::**::::*:.*:::*:**::****:::**:*****:*:*********:*****:********************************:************::*****:*:*:*::*:******:****************:************:******************:***********:*********:**:***:*:**:***::*:*****************:**:**:**.:::***
Consensus	MNAAAKAGKLARAPADLGKGGVPGDAAPGAPGAAPLAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQKEKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTIHELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGVENPLPDRPR---EKEEPVVRETNEAIDCHLSDLLQQLTSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLNYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGTVQINFFQDHTKLILCPLMAAVTYINEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS-