(        10        20        30        40        50        60        70        80        90       100       110       120       130       140       150       160       170       180       190       200       210       220       230       240       250       260       270       280       290       300       310       320       330       340       350       360       370       380       390       400       410       420       430       440       450       460       470       480       490       500       510       520       530       540       550       560       570       580       590       600       610       620       630       640       650       660       670       680       690       700       710       720       730       740       750       760       770       780       790       800       810       820       830       840       850       860       870       880       890       900       910       920       930       940       950       960       970       980       990      1000      1010      1020      1030      1040      1050      1060      1070      1080      1090      1100      1110      1120      1130      1140      1150      1160      1170      1180      1190      1200      1210      1220      1230      1240      1250      1260      1270      1280      1290      1300      )
	|         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |         |
G-alphas human	--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MGCLGNSkT---------------------------EDQRNEEkAQREANKKIEk K28-ac K28-ub QLQkDKQVYRATHRLLLLGAGEsGk K53-me K53-ub STIVkQMRILHVNGFNGEGGEEDPQAARSNSDG-EkATkVQDIkNNLkEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQyFLDk K181-me K181-ub IDVIkQADyVPsDQDLLRCRVLtSGIFEtk K211-me K211-ub FQVDkVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFkSIWNNRWLRTISVILFLNkQDLLAEkVLAGkSkIEDyFPEFARYTTPEDATPEPGEDPRVtRAk K338-ac K338-ub yFIRDEFLrISTAsGDGRHyCyPHFTCAVDTENIRRVFNDCRDIIQRMHLRQyELL
G-alphas iso2 human	--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MGCLGNSKT---------------------------EDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGDS---------------EkATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL
G-alphas iso3 human	--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MGCLGNSKT---------------------------EDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGD----------------EkATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL
G-alphas iso4 human	--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MGCLGNSKT---------------------------EDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGSEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL
G-alphas iso5 human	MGVRNCLYGNNMSGQRDIPPEIGEQPEQPPLEAPGAAAPGAGPSPAEEMETE----PPHNEPIPVENDGEACGPPEVSRPNFQVLNPAFREAGAHGSYSPPPEEAMPFEAEQPSLGGFWPTLEQPGFPSGVHAGLEAFGPALMEPGAFSGARPGLGGYSPPPEEAMPFEFDQPAQRGCSQ-------------LLLQVPDLAPGGPGAAGVPGAPPEEPQALRPAKAGSRGGYSPPPEETMPFELDGEGFGDDSPPPGLSRVIAQVDGSSQFAAVAASSAVRLTPAANAPPLWVPGAIGSPSQEAVRPPSNFTGSSPWMEISGPPFEIGSAPAGVD-DTPVNMDsPPIALDGPPIKVSGAPDKRER---------------A-ERPPVEEEAAEMEGAAD---AAEGGKVPSPGy--G-SPAAGAA-------------------------S------------ADTA------ARA-------------------------APAAPA--------------------D------PDS-----------------G-ATPEDPD-----------------------SGTAPADPDSGAFAADP----------------------------DSGAAPAA-------PADPDSGA---------APDAP------ADPDSGAAPDAPADPDAGAAPEAPAAPAAA------------------------ETRAAHVAPAAP------------D--AGAPTAPAASATRAAQVRRAASAAPAsGARRKIHLRPPsPEIQAADPPtPRPt-RASAWRGKSESSRGRRVYYDEGVASSDDDSSGDESDDGTSGCLRWFQHRRNRRR----RKPQRNLLRNFLVQAFGGCFGRsEsPQPKASRSLKVKKVPLAEKRRQMRKEALEKRAQKRAEKKRSKLIDKQLQDEKMGyMCTHRLLLLGAGESGKSTIVKQMRILHVNGFNGD--------------S-EkATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL
G-alphas iso6 human	MGVRNCLYGNNMSGQRDIPPEIGEQPEQPPLEAPGAAAPGAGPSPAEEMETE----PPHNEPIPVENDGEACGPPEVSRPNFQVLNPAFREAGAHGSYSPPPEEAMPFEAEQPSLGGFWPTLEQPGFPSGVHAGLEAFGPALMEPGAFSGARPGLGGYSPPPEEAMPFEFDQPAQRGCSQ-------------LLLQVPDLAPGGPGAAGVPGAPPEEPQALRPAKAGSRGGYSPPPEETMPFELDGEGFGDDSPPPGLSRVIAQVDGSSQFAAVAASSAVRLTPAANAPPLWVPGAIGSPSQEAVRPPSNFTGSSPWMEISGPPFEIGSAPAGVD-DTPVNMDsPPIALDGPPIKVSGAPDKRER---------------A-ERPPVEEEAAEMEGAAD---AAEGGKVPSPGy--G-SPAAGAA-------------------------S------------ADTA------ARA-------------------------APAAPA--------------------D------PDS-----------------G-ATPEDPD-----------------------SGTAPADPDSGAFAADP----------------------------DSGAAPAA-------PADPDSGA---------APDAP------ADPDSGAAPDAPADPDAGAAPEAPAAPAAA------------------------ETRAAHVAPAAP------------D--AGAPTAPAASATRAAQVRRAASAAPAsGARRKIHLRPPsPEIQAADPPtPRPt-RASAWRGKSESSRGRRVYYDEGVASSDDDSSGDESDDGTSGCLRWFQHRRNRRR----RKPQRNLLRNFLVQAFGGCFGRsEsPQPKASRSLKVKKVPLAEKRRQMRKEALEKRAQKRAEKKRSKLIDKQLQDEKMGyMCTHRLLLLGAGESGKSTIVkQMRILHVNGFNGEGGEEDPQAARSNSDG-EKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRIStAsGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL
G-alphas iso7 human	------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MDRRsRAQQWRRARHNYNDLCPPIGRRAATALLWLSCSIALLRALATSNARAQQRAAAQQRRSFLN-AHHRSG-----------AQVFPESPESESDHEHE----------------------EADLELSLPECLEYEEEFDYETES----ETESEIESE---------TDFETEPETAPTTEP------------ET---EPEDDRGP----VVPKHstFGQsLtQRLHAL--KLRsPDAsPsRAPPstQEPQsPREGEEL--KPEDKDPRDPEESKEPK-----------------EEKQRRRCKPKK-------------------------------------------PT-RrDAsPEsPSKKGPIPIRRH------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
G-alphas iso9 human	-----------------------------------------------MMARPVDPQ-----RSPDPTFRSSTRHSG--KLEPMEATAHLLRKQCPSRLN-----SPAWE----ASGLHWSSLDSPVGSM----QALRPSAQHSWSPEPS------------VVPDQAWEDTALHQKKLCPLSLTSLPREAAVNFSYRSQTLLQEAQVLQGSPELLPRSPKPSGLQRLAPEEATALPLRRLCHLSLMEKDLGtTAHPRGFPELSHKSTAAASSRQSRPRVRSASLPPRtRLPSGsQAPSA----AHPKRLsDLLLt-----------SRAAAPGWRSPDPRSRLAAPPLGSTTLP-STWTAPQSRLTA-RPSRSPEPQIRESE-QRDPQLRRKQQRWKEPLMP----RREEKYPLRGT--DPLPPGQPQRIPLPGQPLQ--------------------------------------------------------------------PQPILTPGQPQKIPT-----PGQHQ---------------------PILTPGHS---QPIPTPGQ-----------PLPPQPIPTPGRPLTPQPIPTPGRPLTPQPIQMPGRPL-----------------RLPPPLRLLRPGQPMSPQLRQtQGLP-LPQPLLPPGQPKsAGRPL-QPLPPGPDARSI-SDPPAPRSRLPIRLLRGLLARLPGGAsPRAAAAAACTT------MKGWPAATMTPAETSPTMGPPDASAGFSIGEIAAAESP-----SATYSATFSCKPSGAASVDLRVPSPKPRAL--------SRSRRYPWRRSADRCAK----------------KPWRsGPRSAQ--RRNAVSSsTNNsRTKRWATCVRTACCF----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
G-alphas mouse	--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MGCLGNSkT---------------------------EDQRNEEkAQREANKKIEKQLQKDKQVYRATHRLLLLGAGEsGkstIVkQMRILHVNGFNGEGGEEDPQAARSNSDG-EKATKVQDIkNNLkEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQyFLDkIDVIkQADYVPSDQDLLRCRVLTsGIFETkFQVDkVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFkSIWNNRWLRTISVILFLNKQDLLAEkVLAGKSkIEDyFPEFARYTtPEDATPEPGEDPRVtRAkYFIRDEFLRIStAsGDGRHYCyPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL
G-alphas iso10 iso4 mouse	MGMFNCLHGNNMSGQHDIPPEVGEQPEQEPLEAPGAAAPGAGAGPAEEMATEPDSEPSNNEPVPDETGSEISGPPEDSKSDIQSPCQAFEEVRVGGDYSPPPEEAMPFETQQPSLGDFWPTLEQPG-PSGTPSGLQAFNPAILEPGTPTGASPGLGAYTPPPEEAMPFEFNEPAQGDHSQ-------------PPLQVPDLAPGGPEAL-VPRALPAEPGNIRFENAGFREDYSPPPEESVPFQVGGEEFGGDSPPPGLPRVIPQIGIGGEFPTVAVPSALCLAPAENAPPLWVRGAIDRPFREAVRSPPNFACDSPPMEITRPLLEIGRASIGVDDDTAVNMDSPPIASDGPPIEVSGAPDKSEC---------------A-ERPPVEREAAEMEGSPTTAT-AVEGKVPSPERGDG-SSTQPEAMDAKPAP---AA------------QA------------VSTG------SDAGAPTDSAMLTD-SQ-SDAGEDG--TAPGTPSD-------------LQSDPEE------LEE-----------------APAVRADPD-----------------------GGAAPVAPATPAES-------------------ESEGSRDPAAEPASEAVPAT-------TAESASGA---------APVTQ------VEPAAAAVSATLAEPAARAAPITPKEPTTRAVPSARAHPAAGAVPGAPAMSASARAAAARAAYAGPLVWGARSLSATPAARASLPARAAAAARAASAARAVAAGRSASAAPSRAHLRPPSPEIQVADPPTPRPPPRPTAWPDKYERGRSCCR-YEASSGICEIESSSDESEEGATGCFQWLLRRNRRPGLPRSHTVGSNPVRNFFTRAFGSCFGLSECTRSRSL-SPGKAKDPMEERRKQMRKEAIEMREQKRADKKRSKLIDKQLEEEKMDYMCTHRLLLLGAGESGKSTIVKQMRILHVNGFNGD--------------S-EKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL
G-alphas iso8 mouse	------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MDRRSRAQQWRRARHNYNDLCPPIGRRAATALLWLSCSIALLRALASSNARAQQRAA--QRRSFLN-AHHRSAAAAAA------AQVLPESSESESDHEHE----------------------EVEPELARPECLEYDQD-DYETETDSETEPESDIESE-----TEIETEPETEPETAPTTEP------------ET---EPEDERGP-------RGATFnQSLTQRLHAL--KLQSADASPRRAQPTTQEPESASEGEEPQRGPLDQDPRDPEEEPEERKE---------------ENRQPRRCKTRR-------------------------------------------PARRRDQsPESPPRKGPIPIRRH------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
G-alphas iso10 mouse	MGMFNCLHGNNMSGQHDIPPEVGEQPEQEPLEAPGAAAPGAGAGPAEEMATEPDSEPSNNEPVPDETGSEISGPPEDSKSDIQSPCQAFEEVRVGGDYSPPPEEAMPFETQQPSLGDFWPTLEQPG-PSGTPSGLQAFNPAILEPGTPTGASPGLGAYTPPPEEAMPFEFNEPAQGDHSQ-------------PPLQVPDLAPGGPEAL-VPRALPAEPGNIRFENAGFREDYSPPPEESVPFQVGGEEFGGDSPPPGLPRVIPQIGIGGEFPTVAVPSALCLAPAENAPPLWVRGAIDRPFREAVRSPPNFACDSPPMEITRPLLEIGRASIGVDDDTAVNMDSPPIASDGPPIEVSGAPDKSEC---------------A-ERPPVEREAAEMEGsPTTAT-AVEGKVPsPERGDG-SSTQPEAMDAKPAP---AA------------QA------------VSTG------SDAGAPTDSAMLTD-SQ-SDAGEDG--TAPGtPsD-------------LQSDPEE------LEE-----------------APAVRADPD-----------------------GGAAPVAPATPAES-------------------ESEGSRDPAAEPASEAVPAT-------TAESASGA---------APVTQ------VEPAAAAVSATLAEPAARAAPITPKEPTTRAVPSARAHPAAGAVPGAPAMSASARAAAARAAYAGPLVWGARsLsATPAARAsLPARAAAAARAASAARAVAAGRSAsAAPSRAHLRPPsPEIQVADPPTPRPPPRPTAWPDKYERGRSCCR-YEASSGICEIESSSDESEEGATGCFQWLLRRNRRPGLPRSHtVGSNPVRNFFTRAFGSCFGLSECTRSRSL-SPGKAKDPMEERRKQMRKEAIEMREQKRADKKRSKLIDKQLEEEKMDYMCTHRLLLLGAGEsGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDG-EKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL
G-alphas iso11 mouse	----------------------------------------MSPSPTRLAVRsVDPQ-----KTPNLTsKAPARPSR--KSEWVETTAHLRRKPCHSRHN-----SPAWE----ISGPPWSSQDH-LGPH----QASKPSTQRFWSPGPP------------LARAQAWEPIPPHQKKLCHLSSTSLPRETIASLPCKSQTLRQEVR-KHWSPELFPRSPGTSDLKTLASEKTTALPLKNLCHFRSVEKNSGAIAHPQDSRESSHKSALAASSRQSRSRVRSASLPPRTRLPSGSEAPLT----DHSARLSDLLLT-----------SHATAPRWRSPDPCLRLAEPPLGSTTTPLSIWTAPQSQVMA-RPSKSREPQIRASA-QRDPQLSEKQPRWKEALP--PPLRWKEKSPLRREG-TDLPPSLKQWM--PSQPLLPKPSL--PDLMLELLRIPRCSQIARAMPEKTGQ---PQ-----ERLQISSRI-------LKNSKKPQLSAPILTEGQPQSPQPLLP-SPSL----KAAEIQPPSQPPRQSLPPRPSLPPGQPLSPRWSPQPRQ-----------SLPPWRSLPPGQPLSPPRSPLPGQSPLLEPIRPLEQSLA-----PQQCQPLLGQLPLGQPMQVHWSGEPGHSQLLPPLGHPFLPAQQLPPGQPLLPAQSLLAGQPLPPPAGPI-LDPPAPRSRLLTRLLRGLLRGRLPGLTSTSGAEAAAGT------RHRLASARSSPPVMSRKKGPPAASSGFCG-ETAALACPGATRSGATQSATSSPEPSEAASVYPSVPDHDPSAP--------GR-PRILWRRGANRCAK----------------KPLRCESRSAQ--IRNAASSSTSNWRRRRWTTCVHTACCF----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
G-alphas rat	--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MGCLGNSKT---------------------------EDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGkSTIVkQMRILHVNGFNGEGGEEDPQAARSNSDG-EKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQyFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETkFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDyFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTAsGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL
G-alphas iso8 rat	------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MDRRSRAHQWRRARHNYNDLCPPIGRRAATALLWLSCSIALLRALASSNARAQQRAA--QRRSFLN-AHHRSAAAAAA------AQVLPESSESESDHEHE----------------------EAEPELARPECLEYDQD-DYETETDSETEPESDIQSE-----TEFETEPETEPETAPTTEP------------ET---EPEDERGP-------RGATFNQSLTQRLHAL--KLQSADASPRRAQPTTQEPESASEGEEPQREPLDEDPRDPEES-EERRE---------------ANRQPRRCKTRR-------------------------------------------PARRRDQSPESPPRKGPIPIRRH------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
G-alphas iso10 rat	MGMLNCLHGNNMSGQHDIPPEVGDQPEQEPLEAQGAAAPGAGVGPAEEMETE----PSNNEPIPDETDSEVCGPPEDSKSDIQSPSQAFEEVQVGGDYSPPPEEAMPFEIQQPSLGDFWPTLEQPG-PSGTPSGIKAFNPAILEPGTPTGAHPGLGAYSPPPEEAMPFEFNEPAQEDRCQ-------------PPLQVPDLAPGGPEAW-VSRALPAEPGNLGFENTGFREDYSPPPEESVPFQLDGEEFGGDSPPPGLPRVTPQIGIGGEFPTVAVPSTLCLAPAANAPPLWVQGAIGRPFREAVRSPN-FAYDISPMEITRPLLEIGRASTGVDDDTAVNMDSPPIASDGPPIEVSGAPVKSEH---------------A-KRPPLERQAAETGNSPISSTTAEEAKVPSLERGEG-SPTQPETVHIKPAP---VA------------ES------------GTDS------SKADP--DSATHAV-LQ-IGPEEVG--GVPTMPTD-------------LP--PAS------EDA-----------------GPDVRAEPD-----------------------GGTAPATPAESEDNREPAAAAAAEPAAEPAAEPAAEPAAEPAAEPAAEAVPDT-------EAESASGA---------VPDTQ------EEPAAAAASATPAEPAARAAPVTPTEPATRAVPSARAHPAAGAVPGASAMSAAARAAAARAAYAGPLVWGARSLSATPAARASLPARAAAAARAASAARAVAAGRSASAAPSRAHLRPPSPEIQVADPPTPRPAPRPSAWPDKYERGRSCCR-YEAASGICEIESSSDESEEGATGCFQWLLRRNRRPGQPRSHTVGSNPVRNFFARAFGSCFGLSECTRSRSL-SPGKAKDPMEERRKQMRKEAMEMREQKRADKKRSKLIDKQLEEEKMDYMCTHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDG-EKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLPRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL
G-alphas iso11 rat	----------------------------------------MSPSPTRLTVRSVDPL-----KTPNLTSRAPVRPSR--KSKWAETTAHLQRKPCHSRSN-----SPAWE----ISGPPWSSLDH-LDPI----RHQSLQPSDFGARTPTGAHPGLGAYSPPPEEAMPFEFNEPAQEDRCQ-------------PPLQVPDLAPGGP-EAWVSRALPAEPGNLGFENTGFREDYSPPPEESVPFQLDGEEFGGDSPPPDSASHATNRHWRFEFPTVAVPSTLCLRPARTRLPSGSRAPLA----DHAR-LSD-LLT-----------SHTTFPQWRSPDPCLRLAEPPLGSTTTPLSIWTAPQSQVMA-RPSKSREPQLRAST-QRDPHLSDKQPRQETALSA-APLQRRQKSPPSSEE-KDPPPNLKQCI--SSQLLLRSPERTLPKPIRTQL----HTQFFRSVLRKSEE---SQPCPPIFRLLLKMRAQMSGQNQTEGQPQPPLPSPKTTENQPPPPPPSQPPSQPLSQPPSQPPSQPPSQLPRQSLTPKPSLPPGQSPTPKRSPQPRQ-----------PLPRRRSLPPGQPPSPLRSPLPGLSLLPEPIQPPGLSLE-----PQRCQPLLGQPPLEQPMQVLWSGEPGHSRLLQPLGHPSLPAQQLPPEQPLLPAQSLPAGQPLPPQAGPI-LDPPARRSRLLTRLLRGLLRGRVPGLTNTNVAEAAAGM------RLRPASARSSPPAMSRKKGPLAASSGFCG-ETAALASPGATQSGATRSATSSPEPSEAASVYLSVPDHDPSAP--------GR-PRILWKRGANRCAK----------------KPWRCESRSAQ--IRNAASSSTSNWRRRRWTTCVHTACCF----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
G-alphas cow	--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MGCLGNSKT---------------------------EDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDG-EKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQDDYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL
	.   ..  ...            .  .  .  ...   .       ... .. . .  .     .....    ...  ..... .  .           ..   .  . .. .... . ...........  ...   ..               ...........    .. . ....       .. .  .......  ..   .. .. ......             .....  .. .. .......... ..      .. . .   .             .......................................           . .  . .... .. .... ....    .   ....... .. ......                          .               .      . .     ..            .     ......                  ..                           ... . ...  .                     ........... .  .                        .. ... . ..         . . . .         ..           ................... ........               .        .....                      . .. ......   . ........  ...   .. .. ......... .. . ...         ..  .  . ...  ..                 .  .   . .    . . ..   .    ....:...:..                           ...:....:.....:.:.:::...:..:..:::::::::::::::::::::::::::::::................ ::::::::::::::::::::::::::::::::::::::::::::::::::::.::::::::::::::::::::::::::::::::::::::::::::::::.:::::::::.::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
Consensus	------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------A------D---N---PPI-----P-----AP-----------------------R-P----A-------------------SP------S-----------------------------------------------------------------------------------P--P-------------------------------------------------------PD---------------------------P--P--P-------------------------------------------------------------------------------PA----------P-AG-----P-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MGCLGNSKT---------------------------EDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGE--------------G-EKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL