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EGLN1 Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Monomer. Interacts with ING4; the interaction inhibits the hydroxylation of HIFs. Interacts with LIMD1. Found in a complex composed of LIMD1, VHL, EGLN1/PHD2, TCEB2 AND CUL2. Interacts with EPAS1. According to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to PubMed:12351678 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle. According to PubMed:12788921; expressed in adult and fetal heart, brain, liver, lung, skeletal muscle and kidney. Also expressed in placenta. Highest levels in adult heart, brain, lung and liver and fetal brain, heart spleen and skeletal muscle. Following exposure to hypoxia, activated in HeLa cells but not in cardiovascular cells. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: EC; Oxidoreductase
Chromosomal Location of Human Ortholog: 1q42.2
Cellular Component: cytoplasm; cytosol; nucleus
Molecular Function: enzyme binding; iron ion binding; L-ascorbic acid binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; peptidyl-proline 4-dioxygenase activity; peptidyl-proline dioxygenase activity; protein binding
Biological Process: cardiac muscle morphogensis; cellular iron ion homeostasis; negative regulation of cAMP catabolic process; negative regulation of cyclic-nucleotide phosphodiesterase activity; negative regulation of transcription factor activity; oxygen homeostasis; peptidyl-proline hydroxylation to 4-hydroxy-L-proline; positive regulation of transcription from RNA polymerase II promoter; regulation of angiogenesis; response to hypoxia
Disease: Erythrocytosis, Familial, 3; Hemoglobin, High Altitude Adaptation
Reference #:  Q9GZT9 (UniProtKB)
Alt. Names/Synonyms: C1orf12; DKFZp761F179; ECYT3; Egl nine homolog 1; egl nine homolog 1 (C. elegans); egl nine-like protein 1; EGLN1; HIF prolyl hydroxylase 2; HIF-PH2; HIF-prolyl hydroxylase 2; HIFPH2; HPH-2; HPH2; Hypoxia-inducible factor prolyl hydroxylase 2; PHD2; Prolyl hydroxylase domain-containing protein 2; SM-20; SM20; zinc finger MYND domain-containing protein 6; ZMYND6
Gene Symbols: EGLN1
Molecular weight: 46,021 Da
Basal Isoelectric point: 8.83  Predict pI for various phosphorylation states
CST Pathways:  Angiogenesis
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