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eNOS endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN. Note: This description may include information from UniProtKB.
Protein type: Amino Acid Metabolism - arginine and proline; EC; Oxidoreductase
Chromosomal Location of Human Ortholog: 7q36.1
Cellular Component: caveola; cytoplasm; cytoskeleton; cytosol; Golgi membrane; nucleus; plasma membrane
Molecular Function: actin monomer binding; arginine binding; cadmium ion binding; calmodulin binding; FAD binding; FMN binding; heme binding; iron ion binding; NADP binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; protein binding; tetrahydrobiopterin binding
Biological Process: angiogenesis; arginine catabolic process; blood vessel remodeling; cell redox homeostasis; endothelial cell migration; in utero embryonic development; lipopolysaccharide-mediated signaling pathway; lung development; mitochondrion organization; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of extrinsic apoptotic signaling pathway via death domain receptors; negative regulation of hydrolase activity; negative regulation of muscle hyperplasia; negative regulation of platelet activation; negative regulation of potassium ion transport; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; ovulation from ovarian follicle; positive regulation of angiogenesis; positive regulation of blood vessel diameter; positive regulation of guanylate cyclase activity; regulation of blood pressure; regulation of blood vessel size; regulation of nitric-oxide synthase activity; regulation of sodium ion transport; regulation of systemic arterial blood pressure by endothelin; regulation of the force of heart contraction by chemical signal; removal of superoxide radicals; response to heat; smooth muscle hyperplasia; vasodilation
Disease: Alzheimer Disease; Hypertension, Essential; Preeclampsia/eclampsia 1; Stroke, Ischemic
Reference #:  P29474 (UniProtKB)
Alt. Names/Synonyms: cNOS; Constitutive NOS; EC-NOS; ECNOS; Endothelial NOS; eNOS; nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase, endothelial; NOS type III; NOS3; NOSIII
Gene Symbols: NOS3
Molecular weight: 133,289 Da
Basal Isoelectric point: 6.94  Predict pI for various phosphorylation states
CST Pathways:  AMPK Signaling  |  Angiogenesis  |  PI3K/Akt Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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