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CPSF3 Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP- dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily. Note: This description may include information from UniProtKB.
Protein type: EC 3.1.27.-; RNA processing; RNA splicing; RNA-binding; Ribonuclease
Chromosomal Location of Human Ortholog: 2p25.1
Cellular Component: intracellular ribonucleoprotein complex; mRNA cleavage and polyadenylation specificity factor complex; nucleoplasm
Molecular Function: 5'-3' exonuclease activity; endoribonuclease activity; metal ion binding; protein binding; RNA binding
Biological Process: histone mRNA 3'-end processing; mRNA 3'-end processing; mRNA cleavage; mRNA export from nucleus; mRNA polyadenylation; mRNA splicing, via spliceosome; termination of RNA polymerase II transcription
Reference #:  Q9UKF6 (UniProtKB)
Alt. Names/Synonyms: cleavage and polyadenylation specific factor 3, 73kDa; cleavage and polyadenylation specificity factor 3; Cleavage and polyadenylation specificity factor 73 kDa subunit; Cleavage and polyadenylation specificity factor subunit 3; CPSF; CPSF 73 kDa subunit; CPSF-73; CPSF3; CPSF73; mRNA 3'-end-processing endonuclease CPSF-73; YSH1
Gene Symbols: CPSF3
Molecular weight: 77,486 Da
Basal Isoelectric point: 5.37  Predict pI for various phosphorylation states
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Protein Structure Not Found.
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