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UBE2N The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Heterodimer with UBE2V2. Interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box domain); the complex has a specific 'Lys-63'-linked polyubiquitination activity. Interacts with RNF8 and RNF168. Interacts with RNF11. Interacts with the E3 ligases, HLTF and SHPRH; the interactions promote the 'Lys-63'-linked polyubiquitination of PCNA upon genotoxic stress and lead to DNA repair. Interacts with ARIH2 (via RING-type 2). Belongs to the ubiquitin-conjugating enzyme family. Note: This description may include information from UniProtKB.
Protein type: DNA repair, damage; EC; Ligase; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 12q22
Cellular Component: cytoplasm; cytosol; fibrillar center; nucleoplasm; nucleus; protein complex; UBC13-MMS2 complex; ubiquitin ligase complex
Molecular Function: protein binding; RNA binding; ubiquitin binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity
Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; DNA double-strand break processing; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; histone ubiquitination; JNK cascade; positive regulation of DNA repair; positive regulation of histone modification; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of ubiquitin-protein ligase activity; postreplication repair; protein modification process; protein ubiquitination; proteolysis; regulation of DNA repair; regulation of histone ubiquitination; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway
Reference #:  P61088 (UniProtKB)
Alt. Names/Synonyms: bendless-like ubiquitin conjugating enzyme; Bendless-like ubiquitin-conjugating enzyme; BLU; MGC131857; MGC8489; Ubc13; UbcH-ben; UBE2N; Ubiquitin carrier protein N; Ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); Ubiquitin-protein ligase N; yeast UBC13 homolog
Gene Symbols: UBE2N
Molecular weight: 17,138 Da
Basal Isoelectric point: 6.13  Predict pI for various phosphorylation states
CST Pathways:  NF-kB Signaling  |  Toll-Like Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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