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CDC7 an evolutionarily conserved serine/threonine protein kinase of the CDC7 family. Predominantly localized in the nucleus, it plays a pivotal role in linking cell cycle regulation to genome duplication, being essential for the firing of DNA replication origins. Plays an essential role in initiating DNA replication at replication origins by phosphorylating the MCM2 and MCM4 subunits of the MCM2-7 helicase complex. The human protein requires phosphorylation by CDK1 at T376 and binding to activator of S-phase kinase (ASK)/DBF4B to be fully activated. The interaction between Cdc7 and DBFK subunits facilitates ATP binding and substrate recognition by the Cdc7 kinase. Although CDC7 expression levels appear to be constant throughout the cell cycle, the expression levels of DBF4B is cell cycle-regulated. Thus, the CDC7 activity is activated during S phase in eukaryotes. The Cdc7/ DBF4B complex regulates initiation of DNA replication by phosphorylating chromatin-bound mini-chromosome maintenance protein 2 (MCM2). Impaired Cdc7 kinase activity leads to S-phase arrest. Plays a role in the TGF-beta regulation of smooth muscle cell (SMC) differentiation. Interacts with Smad3 to facilitate Smad3 binding to SMC marker promoter, leading to the activation of SMC marker gene transcription. Note: This description may include information from UniProtKB.
Protein type: CDC7 family; EC; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 1p22.1
Cellular Component: cytoplasm; intercellular bridge; nucleoplasm; nucleus
Molecular Function: kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity
Biological Process: DNA replication; DNA replication initiation; double-strand break repair via break-induced replication; G1/S transition of mitotic cell cycle; peptidyl-serine phosphorylation; phagocytosis; positive regulation of cell proliferation; regulation of cell shape
Reference #:  O00311 (UniProtKB)
Alt. Names/Synonyms: CDC7; CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1; CDC7-related kinase; CDC7L1; cell division cycle 7 homolog (S. cerevisiae); cell division cycle 7-like protein 1; Cell division cycle 7-related protein kinase; DDK; HsCdc7; Hsk1; huCdc7; MGC117361; MGC126237; MGC126238
Gene Symbols: CDC7
Molecular weight: 63,888 Da
Basal Isoelectric point: 8.96  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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