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ROCK1 Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Homodimer. Interacts with RHOB, RHOC, MYLC2B snd PTEN. Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner. Detected in blood platelets. Activated by RHOA binding. Inhibited by Y- 27632. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. Note: This description may include information from UniProtKB.
Protein type: AGC group; DMPK family; EC; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); ROCK subfamily
Chromosomal Location of Human Ortholog: 18q11.1
Cellular Component: cytoskeleton; cytosol; extracellular region; lamellipodium; plasma membrane; ruffle
Molecular Function: protein binding; protein kinase activity; protein serine/threonine kinase activity
Biological Process: actin cytoskeleton organization and biogenesis; cortical actin cytoskeleton organization and biogenesis; ephrin receptor signaling pathway; I-kappaB kinase/NF-kappaB cascade; leukocyte adhesion; leukocyte migration; leukocyte tethering or rolling; membrane to membrane docking; myoblast migration; negative regulation of angiogenesis; negative regulation of protein binding; neutrophil degranulation; positive regulation of focal adhesion formation; regulation of actin cytoskeleton organization and biogenesis; regulation of cell adhesion; regulation of focal adhesion formation; regulation of keratinocyte differentiation; regulation of stress fiber formation; signal transduction; smooth muscle contraction; vascular endothelial growth factor receptor signaling pathway
Reference #:  Q13464 (UniProtKB)
Alt. Names/Synonyms: MGC131603; MGC43611; p160 ROCK-1; p160-ROCK; p160ROCK; PRO0435; Renal carcinoma antigen NY-REN-35; Rho kinase; Rho-associated protein kinase 1; Rho-associated, coiled-coil containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase 1; ROCK1
Gene Symbols: ROCK1
Molecular weight: 158,175 Da
Basal Isoelectric point: 5.67  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  Adherens Junction Dynamics  |  Apoptosis Regulation  |  Death Receptor Signaling  |  Microtubule Dynamics  |  TGF-ß Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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