required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks. Interacts with CHEK1 when phosphorylated. Interacts with ATR and RAD9A and these interactions are slightly reduced during checkpoint activation. Interacts with BRCA1 and this interaction increases during checkpoint activation. Note: This description may include information from UniProtKB.
Protein type: Actin-binding; Cell cycle regulation
Biological Process: activation of protein kinase activity; DNA damage checkpoint; DNA replication; G2/M transition DNA damage checkpoint; mitotic cell cycle DNA replication checkpoint; peptidyl-serine phosphorylation; protein deubiquitination