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Diaphanous-1 Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration. Has neurite outgrowth promoting activity. In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA- DIAPH1 signaling pathway plays an important role in ERBB2- dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. Homodimer. Interacts with the GTP-bound form of RHOA. Interacts with RHOC, PFY1, MAPRE1, BAIAP2 and APC. Interacts with SCAI. Interacts with DCAF7, via FH2 domain. Interacts with NCDN. Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea. Belongs to the formin homology family. Diaphanous subfamily. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Actin-binding; Adaptor/scaffold; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 5q31.3
Cellular Component: cytosol; plasma membrane; secretory granule membrane
Molecular Function: protein binding; RNA binding
Biological Process: cytoskeleton organization and biogenesis; neutrophil degranulation; regulation of cell shape; regulation of microtubule-based process; regulation of release of sequestered calcium ion into cytosol
Disease: Deafness, Autosomal Dominant 1; Seizures, Cortical Blindness, And Microcephaly Syndrome
Reference #:  O60610 (UniProtKB)
Alt. Names/Synonyms: DFNA1; DIA1; DIAP1; DIAPH1; diaphanous homolog 1 (Drosophila); diaphanous-related formin 1; Diaphanous-related formin-1; DRF1; FLJ25265; hDIA1; LFHL1; Protein diaphanous homolog 1
Gene Symbols: DIAPH1
Molecular weight: 141,347 Da
Basal Isoelectric point: 5.31  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  Adherens Junction Dynamics  |  Microtubule Dynamics  |  TGF-ß Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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