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IRE1 a ser/thr protein kinase that possess endonuclease activity. Important in altering gene expression as a response to endoplasmic reticulum based stress signals. Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain, which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain. Ubiquitously expressed. High levels observed in pancreatic tissue. Functionally connected with insulin biosynthesis in pancreatic beta cells. Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of IRE1. Note: This description may include information from UniProtKB.
Protein type: Apoptosis; Chaperone; EC; Endoplasmic reticulum; IRE family; Kinase, protein; Membrane protein, integral; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Ribonuclease
Chromosomal Location of Human Ortholog: 17q23.3
Cellular Component: cytoplasm; endoplasmic reticulum; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane
Molecular Function: ADP binding; ATP binding; endoribonuclease activity; enzyme binding; Hsp70 protein binding; Hsp90 protein binding; identical protein binding; magnesium ion binding; platelet-derived growth factor receptor binding; protein binding; protein homodimerization activity; protein serine/threonine kinase activity
Biological Process: activation of JNK activity; cell cycle arrest; endothelial cell proliferation; mRNA catabolic process; mRNA cleavage; positive regulation of RNA splicing; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of macroautophagy; unfolded protein response, activation of signaling protein activity
Reference #:  O75460 (UniProtKB)
Alt. Names/Synonyms: endoplasmic reticulum to nucleus signaling 1; endoplasmic reticulum to nucleus signalling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signalling 1; ERN1; FLJ30999; hIRE1p; inositol-requiring 1; Inositol-requiring protein 1; IRE1; Ire1-alpha; IRE1a; IRE1P; MGC163277; MGC163279; protein kinase/endoribonuclease; Serine/threonine-protein kinase; Serine/threonine-protein kinase/endoribonuclease IRE1
Gene Symbols: ERN1
Molecular weight: 109,735 Da
Basal Isoelectric point: 5.98  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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