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CAD a multifunctional enzyme that catalyzes four enzymatic activities of the pyrimidine pathway, including the rate-limiting first step. The four enzymatic activities involved in de novo pyrimidine synthesis are glutamine amidotransferase, carbamoyl-phosphate synthetase, aspartate transcarbamoylase, and dihydroorotase activities. Provides essential precursors for DNA synthesis. Required for the de novo synthesis of pyrimidine nucleotides and cell growth. Phosphorylation by p70S6K promotes CAD oligomerization, stimulating de novo synthesis of pyrimidines and progression through S phase in mammalian cells. Utilizes glutamine, bicarbonate, and aspartic acid to form a pyrimidine ring that is subsequently attached to ribose to generate a pyrimidine. Note: This description may include information from UniProtKB.
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; EC; EC; EC; Hydrolase; Ligase; Nucleotide Metabolism - pyrimidine; Transferase
Chromosomal Location of Human Ortholog: 2p23.3
Cellular Component: cell projection; cell soma; cytosol; membrane; nuclear matrix; nucleoplasm; nucleus; terminal button
Molecular Function: aspartate carbamoyltransferase activity; ATP binding; carbamoyl-phosphate synthase (ammonia) activity; carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; dihydroorotase activity; enzyme binding; identical protein binding; protein kinase activity; zinc ion binding
Biological Process: 'de novo' pyrimidine base biosynthetic process; arginine biosynthetic process; drug metabolic process; glutamine metabolic process; peptidyl-threonine phosphorylation; protein amino acid autophosphorylation; pyrimidine nucleoside biosynthetic process; urea cycle
Disease: Congenital Disorder Of Glycosylation, Type Iz
Reference #:  P27708 (UniProtKB)
Alt. Names/Synonyms: Aspartate carbamoyltransferase; CAD; CAD protein; CAD trifunctional protein; carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase; Dihydroorotase; Glutamine-dependent carbamoyl-phosphate synthase; multifunctional protein CAD; PYR1
Gene Symbols: CAD
Molecular weight: 242,984 Da
Basal Isoelectric point: 6.02  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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