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NANOG Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cells to enter into S phase and proliferation. Interacts with SMAD1 and SALL4. Expressed in testicular carcinoma and derived germ cell tumors. Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Belongs to the Nanog homeobox family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Cell development/differentiation; DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 6|6 F1
Cellular Component: condensed chromosome; nucleolus; nucleoplasm; nucleus
Molecular Function: chromatin binding; DNA binding; DNA binding transcription factor activity; protein binding; sequence-specific DNA binding; transcription corepressor activity; transcription factor binding
Biological Process: cell dedifferentiation; cell proliferation; embryonic development; embryonic pattern specification; endodermal cell fate specification; gonad development; mesodermal cell fate commitment; multicellular organism development; negative regulation of BMP signaling pathway; negative regulation of cell differentiation; negative regulation of cell fate commitment; negative regulation of endodermal cell fate specification; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell proliferation; positive regulation of mitotic cell cycle; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of cell differentiation; regulation of gene expression; regulation of transcription, DNA-templated; response to organic substance; response to retinoic acid; somatic stem cell maintenance; stem cell differentiation; stem cell division; stem cell maintenance; transcription, DNA-dependent
Reference #:  Q80Z64 (UniProtKB)
Alt. Names/Synonyms: 2410002E02Rik; early embryo specific expression NK family; ecat4; ENK; homeobox protein NANOG; homeobox transcription factor Nanog; Nanog; Nanog homeobox; Q5TM83; Q80Z64; Q80Z64-2
Gene Symbols: Nanog
Molecular weight: 34,194 Da
Basal Isoelectric point: 6.32  Predict pI for various phosphorylation states
CST Pathways:  ESC Pluripotency and Differentiation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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