TOPBP1 a DNA-binding protein and scaffolding protein that is required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR. Contains 7 BRCT domains, which are characteristic of proteins involved in cell cycle checkpoint control in response to DNA damage. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase. Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Up-regulated during the S phase of the cell cycle. Up-regulated by E2F1 and interferon. Note: This description may include information from UniProtKB.
Protein type: DNA replication
Chromosomal Location of Human Ortholog: 9|9 F1
Cellular Component:  actin cytoskeleton; chromosome; condensed nuclear chromosome; cytoplasm; cytoskeleton; male germ cell nucleus; nuclear body; nucleus; PML body
Molecular Function:  DNA binding; identical protein binding
Biological Process:  cellular response to DNA damage stimulus; DNA repair; mitotic DNA replication checkpoint; reciprocal meiotic recombination; response to ionizing radiation
Reference #:  Q6ZQF0 (UniProtKB)
Alt. Names/Synonyms: 1110031N14Rik; 2810429C13Rik; AI256758; D430026L04Rik; DNA topoisomerase 2-binding protein 1; DNA topoisomerase II-beta-binding protein 1; DNA topoisomerase II-binding protein 1; Kiaa0259; mKIAA0259; TOPB1; TopBP1; topoisomerase (DNA) II beta binding protein; topoisomerase (DNA) II binding protein 1
Gene Symbols: Topbp1
Molecular weight: 168,860 Da
Basal Isoelectric point: 6.22  Predict pI for various phosphorylation states
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Protein Structure Not Found.

Cross-references to other databases:  STRING  |  BioGPS  |  Pfam  |  RCSB PDB  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene