HEAB
Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Its role in tRNA splicing and maturation is required for cerebellar development. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing. Belongs to the Clp1 family. Clp1 subfamily. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
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Protein type: EC 2.7.1.78; RNA splicing; RNA-binding |
Chromosomal Location of mouse Ortholog: 2|2 D |
Cellular Component:
cytosol; mRNA cleavage factor complex; nucleoplasm; nucleus; tRNA-intron endonuclease complex
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Molecular Function:
ATP binding; ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; ATP-dependent polynucleotide 5'-hydroxyl-kinase activity; ATP-dependent polyribonucleotide 5'-hydroxyl-kinase activity; kinase activity; nucleotide binding; polynucleotide 5'-hydroxyl-kinase activity; transferase activity
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Biological Process:
cerebellar cortex development; global gene silencing by mRNA cleavage; mRNA 3'-end processing; mRNA polyadenylation; mRNA processing; phosphorylation; RISC complex assembly; RNA processing; tRNA processing; tRNA splicing, via endonucleolytic cleavage and ligation
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Reference #:
Q99LI9
(UniProtKB)
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Alt. Names/Synonyms: AI462438; ATP/GTP-binding protein; Clp1; CLP1, cleavage and polyadenylation factor I subunit; CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae); Heab; OTTMUSP00000014630; Polyadenylation factor Clp1; Polynucleotide kinase Clp1; Polyribonucleotide 5'-hydroxyl-kinase Clp1; Pre-mRNA cleavage complex II protein Clp1
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Gene Symbols: Clp1
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Molecular weight:
47,738 Da
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Basal Isoelectric point:
6.2
Predict pI for various phosphorylation states
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