HEAB Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Its role in tRNA splicing and maturation is required for cerebellar development. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing. Belongs to the Clp1 family. Clp1 subfamily. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: EC; RNA splicing; RNA-binding
Chromosomal Location of Human Ortholog: 2|2 D
Cellular Component:  cytosol; mRNA cleavage factor complex; nucleoplasm; nucleus; tRNA-intron endonuclease complex
Molecular Function:  ATP binding; ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; ATP-dependent polyribonucleotide 5'-hydroxyl-kinase activity; kinase activity; nucleotide binding; polydeoxyribonucleotide kinase activity; transferase activity
Biological Process:  cerebellar cortex development; mRNA 3'-end processing; mRNA polyadenylation; mRNA processing; phosphorylation; siRNA loading onto RISC involved in RNA interference; targeting of mRNA for destruction involved in RNA interference; tRNA processing; tRNA splicing, via endonucleolytic cleavage and ligation
Reference #:  Q99LI9 (UniProtKB)
Alt. Names/Synonyms: AI462438; ATP/GTP-binding protein; Clp1; CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae); Heab; OTTMUSP00000014630; Polynucleotide kinase Clp1; Polyribonucleotide 5'-hydroxyl-kinase Clp1; Pre-mRNA cleavage complex II protein Clp1
Gene Symbols: Clp1
Molecular weight: 47,738 Da
Basal Isoelectric point: 6.2  Predict pI for various phosphorylation states
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Protein Structure Not Found.

Cross-references to other databases:  STRING  |  Reactome  |  BioGPS  |  Pfam  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene