METTL16 RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts. Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure. Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing and/or stability of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, impairing MAT2A expression. In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A. In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs. Also able to bind various lncRNAs. Specifically binds the 3'-end of the MALAT1 long non-coding RNA. Belongs to the methyltransferase superfamily. METTL16/RlmF family. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: EC 2.1.1.-; Methyltransferase
Chromosomal Location of Human Ortholog: 17p13.3
Cellular Component:  nucleus
Molecular Function:  mRNA (N6-adenosine)-methyltransferase activity; RNA binding; RNA stem-loop binding; U6 snRNA (adenine-(43)-N(6))-methyltransferase activity; U6 snRNA 3'-end binding
Biological Process:  mRNA catabolic process; mRNA destabilization; mRNA methylation; posttranscriptional regulation of gene expression; regulation of mRNA splicing, via spliceosome; S-adenosylmethionine biosynthetic process; snRNA (adenine-N6)-methylation
Reference #:  Q86W50 (UniProtKB)
Alt. Names/Synonyms: MALAT1; MET10; methyltransferase 10 domain containing; Methyltransferase 10 domain-containing protein; METT10D; MGC3329; Putative methyltransferase METT10D; U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase
Gene Symbols: METTL16
Molecular weight: 63,621 Da
Basal Isoelectric point: 8.08  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  STRING  |  cBioPortal  |  Wikipedia  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  InnateDB