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METTL16
RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts. Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure. Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing and/or stability of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, impairing MAT2A expression. In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A. In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs. Also able to bind various lncRNAs. Specifically binds the 3'-end of the MALAT1 long non-coding RNA. Belongs to the methyltransferase superfamily. METTL16/RlmF family. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
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| Protein type: EC 2.1.1.-; Methyltransferase |
| Chromosomal Location of human Ortholog: 17p13.3 |
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Cellular Component:
cytoplasm; nucleus
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Molecular Function:
23S rRNA (adenine(1618)-N(6))-methyltransferase activity; mRNA (N6-adenosine)-methyltransferase activity; RNA binding; RNA stem-loop binding; U6 snRNA (adenine-(43)-N(6))-methyltransferase activity; U6 snRNA 3'-end binding
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Biological Process:
mRNA catabolic process; mRNA destabilization; mRNA methylation; negative regulation of 3'-UTR-mediated mRNA stabilization; post-transcriptional regulation of gene expression; regulation of mRNA splicing, via spliceosome; rRNA base methylation; S-adenosylmethionine biosynthetic process; snRNA (adenine-N6)-methylation
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Reference #:
Q86W50
(UniProtKB)
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Alt. Names/Synonyms: MALAT1; MET10; MET16; methyltransferase 10 domain containing; Methyltransferase 10 domain-containing protein; methyltransferase like 16; Methyltransferase-like protein 16; METT10D; METTL16; MGC3329; N6-adenosine-methyltransferase METTL16; Putative methyltransferase METT10D; RNA N6-adenosine-methyltransferase METTL16; U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase; U6 snRNA methyltransferase
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Gene Symbols: METTL16
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Molecular weight:
63,621 Da
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Basal Isoelectric point:
8.08
Predict pI for various phosphorylation states
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Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology®
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