RFWD3 E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage. Plays a key role in RPA-mediated DNA damage signaling and repair. Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination. Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. Note: This description may include information from UniProtKB.
Protein type: EC 6.3.2.-; EC; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 16q23.1
Cellular Component:  cytoplasm; nucleoplasm; nucleus; PML body; site of DNA damage
Molecular Function:  MDM2/MDM4 family protein binding; metal ion binding; p53 binding; protein binding; ubiquitin-protein transferase activity
Biological Process:  cellular response to DNA damage stimulus; chromosome breakage; double-strand break repair via homologous recombination; interstrand cross-link repair; mitotic G1 DNA damage checkpoint; protein ubiquitination; regulation of DNA damage checkpoint; replication fork processing; response to ionizing radiation
Disease: Fanconi Anemia, Complementation Group W
Reference #:  Q6PCD5 (UniProtKB)
Alt. Names/Synonyms: E3 ubiquitin-protein ligase RFWD3; FLJ10520; RFWD3; ring finger and WD repeat domain 3; RING finger and WD repeat domain-containing protein 3; RING finger protein 201; RNF201
Gene Symbols: RFWD3
Molecular weight: 85,094 Da
Basal Isoelectric point: 6.02  Predict pI for various phosphorylation states
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Protein Structure Not Found.

Cross-references to other databases:  STRING  |  cBioPortal  |  Wikipedia  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene