eNOS endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN. Note: This description may include information from UniProtKB.
Protein type: Amino Acid Metabolism - arginine and proline; EC; Oxidoreductase
Chromosomal Location of Human Ortholog: 7q36.1
Cellular Component:  caveola; cytoplasm; cytoskeleton; cytosol; endocytic vesicle membrane; Golgi apparatus; Golgi membrane; nucleus; plasma membrane
Molecular Function:  actin monomer binding; arginine binding; cadmium ion binding; calmodulin binding; flavin adenine dinucleotide binding; FMN binding; heme binding; NADP binding; nitric-oxide synthase activity; protein binding; scaffold protein binding; tetrahydrobiopterin binding
Biological Process:  angiogenesis; aortic valve morphogenesis; arginine catabolic process; blood vessel remodeling; cell redox homeostasis; cofactor metabolic process; endocardial cushion morphogenesis; endothelial cell migration; homeostasis of number of cells within a tissue; in utero embryonic development; lipopolysaccharide-mediated signaling pathway; lung development; mitochondrion organization; negative regulation of biomineral tissue development; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of extrinsic apoptotic signaling pathway via death domain receptors; negative regulation of hydrolase activity; negative regulation of muscle hyperplasia; negative regulation of platelet activation; negative regulation of potassium ion transport; nitric oxide biosynthetic process; ovulation from ovarian follicle; oxidation-reduction process; positive regulation of angiogenesis; positive regulation of blood vessel endothelial cell migration; positive regulation of gene expression; positive regulation of guanylate cyclase activity; pulmonary valve morphogenesis; regulation of blood pressure; regulation of blood vessel size; regulation of neurological system process; regulation of nitric-oxide synthase activity; regulation of sodium ion transport; regulation of systemic arterial blood pressure by endothelin; regulation of the force of heart contraction by chemical signal; removal of superoxide radicals; response to fluid shear stress; response to heat; smooth muscle hyperplasia; vasodilation; ventricular septum morphogenesis
Disease: Alzheimer Disease; Hypertension, Essential; Preeclampsia/eclampsia 1; Stroke, Ischemic
Reference #:  P29474 (UniProtKB)
Alt. Names/Synonyms: cNOS; Constitutive NOS; EC-NOS; ECNOS; Endothelial NOS; eNOS; nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase, endothelial; NOS type III; NOS3; NOSIII
Gene Symbols: NOS3
Molecular weight: 133,289 Da
Basal Isoelectric point: 6.94  Predict pI for various phosphorylation states
CST Pathways:  AMPK Signaling  |  Angiogenesis  |  PI3K/Akt Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  STRING  |  cBioPortal  |  Wikipedia  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene