RPA1 As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. Belongs to the replication factor A protein 1 family. Note: This description may include information from UniProtKB.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 11 B5|11 45.79 cM
Cellular Component:  condensed chromosome; condensed nuclear chromosome; DNA replication factor A complex; lateral element; male germ cell nucleus; nuclear chromosome, telomeric region; nucleoplasm; nucleus; PML body; site of DNA damage
Molecular Function:  chromatin binding; damaged DNA binding; DNA binding; G-rich strand telomeric DNA binding; metal ion binding; nucleic acid binding; sequence-specific DNA binding; single-stranded DNA binding; single-stranded telomeric DNA binding
Biological Process:  base-excision repair; cellular response to DNA damage stimulus; chromosome organization; DNA recombination; DNA repair; DNA replication; DNA unwinding involved in DNA replication; DNA-dependent DNA replication; double-strand break repair via homologous recombination; hemopoiesis; homeostasis of number of cells within a tissue; in utero embryonic development; meiotic cell cycle; mismatch repair; nucleotide-excision repair; positive regulation of cell proliferation; protein localization to chromosome; telomere maintenance; telomere maintenance via telomerase
Reference #:  Q8VEE4 (UniProtKB)
Alt. Names/Synonyms: 5031405K23Rik; 70kDa; AA589576; AW557552; OTTMUSP00000006492; replication factor A protein 1; Replication protein A 70 kDa DNA-binding subunit; Replication protein A1; RF-A; RF-A protein 1; RP-A; RP-A p70; Rpa; Rpa1
Gene Symbols: Rpa1
Molecular weight: 69,037 Da
Basal Isoelectric point: 8.13  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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RPA1

Protein Structure Not Found.


Cross-references to other databases:  STRING  |  Reactome  |  BioGPS  |  Pfam  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein