FEN1 Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily. 2 alternative initiation human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: DNA repair, damage; DNA-binding; Deoxyribonuclease; EC 3.1.-.-; Nuclear receptor co-regulator; Nucleolus; Ribonuclease
Chromosomal Location of Human Ortholog: 11q12.2
Cellular Component:  mitochondrion; nucleolus; nucleoplasm; nucleus; protein-containing complex
Molecular Function:  5'-3' exonuclease activity; 5'-flap endonuclease activity; damaged DNA binding; DNA binding; double-stranded DNA binding; double-stranded DNA exodeoxyribonuclease activity; endonuclease activity; exonuclease activity; flap endonuclease activity; protein binding; RNA-DNA hybrid ribonuclease activity
Biological Process:  base-excision repair; DNA repair; DNA replication; DNA replication, removal of RNA primer; double-strand break repair; double-strand break repair via homologous recombination; memory; nucleic acid phosphodiester bond hydrolysis; positive regulation of sister chromatid cohesion; RNA phosphodiester bond hydrolysis, endonucleolytic; telomere maintenance via semi-conservative replication; UV protection
Reference #:  P39748 (UniProtKB)
Alt. Names/Synonyms: DNase IV; FEN-1; FEN1; Flap endonuclease 1; Flap structure-specific endonuclease 1; hFEN-1; Maturation factor 1; maturation factor-1; MF1; RAD2
Gene Symbols: FEN1
Molecular weight: 42,593 Da
Basal Isoelectric point: 8.8  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

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