Pumilio-2
Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation. Plays a role in cytoplasmic sensing of viral infection. Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm. May regulate DCUN1D3 mRNA levels. May support proliferation and self-renewal of stem cells. Binds specifically to miRNA MIR199A precursor, with PUM1, regulates miRNA MIR199A expression at a postranscriptional level. Expressed in male germ cells of adult testis (at protein level). Highly expressed in testis and ovary. Predominantly expressed in stem cells and germ cells. Expressed at lower level in brain, heart, kidney, liver, muscle, placenta, intestine and stomach Expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested. 4 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: Motility/polarity/chemotaxis; RNA-binding; Translation
Chromosomal Location of mouse Ortholog: 12|12 A1.1
Cellular Component: cytoplasm; cytoplasmic stress granule; cytosol; dendrite; neuronal cell body; nuclear membrane; perinuclear region of cytoplasm
Molecular Function: lncRNA binding; miRNA binding; mRNA 3'-UTR binding; protein binding; pumilio-response element binding; RNA binding
Biological Process: adipose tissue development; chromosome organization; hair follicle development; miRNA processing; mitochondrion organization; negative regulation of gene expression; positive regulation of miRNA-mediated gene silencing; positive regulation of RIG-I signaling pathway; positive regulation of sprouting of injured axon; post-transcriptional regulation of gene expression; regulation of chromosome segregation; regulation of intracellular mRNA localization; regulation of mRNA stability; regulation of translation; respiratory electron transport chain; skeletal system development; stress granule assembly
