VPS35 Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The CSC seems to associate with the cytoplasmic domain of cargo proteins predominantly via VPS35; however, these interactions seem to be of low affinity and retromer SNX proteins may also contribute to cargo selectivity thus questioning the classical function of the CSC. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5. Required for retrograde transport of lysosomal enzyme receptor IGF2R and SLC11A2. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Required for endosomal localization of WASHC2C. Mediates the association of the CSC with the WASH complex via WASHC2. Required for the endosomal localization of TBC1D5. (Microbial infection) The heterotrimeric retromer cargo-selective complex (CSC) mediates the exit of human papillomavirus from the early endosome and the delivery to the Golgi apparatus. Belongs to the VPS35 family. Ubiquitous. Highly expressed in heart, brain, placenta, skeletal muscle, spleen, thymus, testis, ovary, small intestine, kidney and colon. Note: This description may include information from UniProtKB.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 16q11.2
Cellular Component:  cytosol; early endosome; endosome; endosome membrane; glutamatergic synapse; lysosome; mitochondrion-derived vesicle; neuron projection; neuronal cell body; perinuclear region of cytoplasm; plasma membrane; postsynaptic density; presynapse; retromer complex; retromer, cargo-selective complex; tubular endosome
Molecular Function:  D1 dopamine receptor binding; protein binding
Biological Process:  lysosome organization; mitochondrial fragmentation involved in apoptotic process; mitochondrion to lysosome transport; negative regulation of cell death; negative regulation of cellular protein localization; negative regulation of gene expression; negative regulation of inflammatory response; negative regulation of late endosome to lysosome transport; negative regulation of lysosomal protein catabolic process; negative regulation of neuron death; negative regulation of protein homooligomerization; neurotransmitter receptor transport, endosome to plasma membrane; neurotransmitter receptor transport, endosome to postsynaptic membrane; positive regulation of canonical Wnt signaling pathway; positive regulation of cellular protein catabolic process; positive regulation of dopamine biosynthetic process; positive regulation of dopamine receptor signaling pathway; positive regulation of gene expression; positive regulation of locomotion involved in locomotory behavior; positive regulation of mitochondrial fission; positive regulation of Wnt protein secretion; protein destabilization; protein localization to endosome; regulation of cellular protein metabolic process; regulation of dendritic spine maintenance; regulation of macroautophagy; regulation of mitochondrion organization; regulation of presynapse assembly; regulation of protein stability; regulation of terminal button organization; retrograde transport, endosome to Golgi; retrograde transport, endosome to plasma membrane; synapse assembly; transcytosis; vesicle-mediated transport in synapse; viral process; voluntary musculoskeletal movement; Wnt signaling pathway
Disease: Parkinson Disease 17
Reference #:  Q96QK1 (UniProtKB)
Alt. Names/Synonyms: DKFZp434E1211; DKFZp434P1672; FLJ10752; FLJ13588; FLJ20388; hVPS35; Maternal-embryonic 3; MEM3; PARK17; vacuolar protein sorting 35 homolog; vacuolar protein sorting 35 homolog (S. cerevisiae); Vacuolar protein sorting-associated protein 35; Vesicle protein sorting 35; VPS35; VPS35 retromer complex component
Gene Symbols: VPS35
Molecular weight: 91,707 Da
Basal Isoelectric point: 5.32  Predict pI for various phosphorylation states
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  STRING  |  cBioPortal  |  Wikipedia  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene