PRPF8 Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site. Widely expressed. Note: This description may include information from UniProtKB.
Protein type: RNA splicing; RNA-binding; Spliceosome
Chromosomal Location of human Ortholog: 17p13.3
Cellular Component:  catalytic step 2 spliceosome; nuclear speck; nucleoplasm; nucleus; U2-type catalytic step 2 spliceosome; U2-type precatalytic spliceosome; U4/U6 x U5 tri-snRNP complex
Molecular Function:  K63-linked polyubiquitin modification-dependent protein binding; protein binding
Biological Process:  cellular response to lipopolysaccharide; cellular response to tumor necrosis factor; mRNA processing; mRNA splicing, via spliceosome; RNA splicing; RNA splicing, via transesterification reactions; spliceosomal tri-snRNP complex assembly
Disease: Retinitis Pigmentosa 13
Reference #:  Q6P2Q9 (UniProtKB)
Alt. Names/Synonyms: 220 kDa U5 snRNP-specific protein; apoptosis-regulated protein 1; apoptosis-regulated protein 2; HPRP8; p220; pre-mRNA processing factor 8; Pre-mRNA-processing-splicing factor 8; precursor mRNA processing protein; PRP8; PRP8 homolog; PRP8 pre-mRNA processing factor 8 homolog; PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae); PRPC8; PRPF8; RP13; SNRNP220; Splicing factor Prp8; U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p
Gene Symbols: PRPF8
Molecular weight: 273,600 Da
Basal Isoelectric point: 8.95  Predict pI for various phosphorylation states
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Protein Structure Not Found.

Cross-references to other databases:  AlphaFold  |  STRING  |  cBioPortal  |  Wikipedia  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  InnateDB