Wee1 Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.10.2; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); WEE family
Chromosomal Location of Human Ortholog: 11p15.4
Cellular Component:  cytoplasm; nucleolus; nucleoplasm
Molecular Function:  ATP binding; kinase activity; magnesium ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding
Biological Process:  cell division; establishment of cell polarity; G2/M transition of mitotic cell cycle; microtubule cytoskeleton organization; negative regulation of G1/S transition of mitotic cell cycle; neuron projection morphogenesis; peptidyl-tyrosine phosphorylation
Reference #:  P30291 (UniProtKB)
Alt. Names/Synonyms: DKFZp686I18166; FLJ16446; WEE1; WEE1 G2 checkpoint kinase; Wee1-like protein kinase; WEE1A; Wee1A kinase; WEE1hu
Gene Symbols: WEE1
Molecular weight: 71,597 Da
Basal Isoelectric point: 6.33  Predict pI for various phosphorylation states
CST Pathways:  G2/M DNA Damage Checkpoint  |  PI3K/Akt Signaling
Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology® Total Proteins
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Wee1

Protein Structure Not Found.


Cross-references to other databases:  STRING  |  cBioPortal  |  Wikipedia  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  KinBase  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene