Wee1 Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.

Protein type: EC 2.7.10.2; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Protein kinase, tyrosine (non-receptor); WEE family; WEE1 subfamily

Chromosomal Location of human Ortholog: 11p15.4

Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus

Molecular Function: ATP binding; magnesium ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein tyrosine kinase activity

Biological Process: cell division; establishment of cell polarity; G2/M transition of mitotic cell cycle; microtubule cytoskeleton organization; negative regulation of G1/S transition of mitotic cell cycle; neuron projection morphogenesis; peptidyl-tyrosine phosphorylation; protein phosphorylation

Reference #: P30291 (UniProtKB)

Alt. Names/Synonyms: DKFZp686I18166; FLJ16446; WEE1; WEE1 G2 checkpoint kinase; WEE1 homolog; WEE1+ homolog; Wee1-like protein kinase; WEE1A; Wee1A kinase; WEE1hu

Gene Symbols: WEE1

Molecular weight: 71,597 Da

Basal Isoelectric point: 6.33 Predict pI for various phosphorylation states

CST Pathways: G2/M DNA Damage Checkpoint | PI3K/Akt Signaling

Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology^®

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Wee1

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