PCNA This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family. Note: This description may include information from UniProtKB.

Protein type: Cell cycle regulation; DNA replication

Chromosomal Location of Human Ortholog: 20p12.3

Cellular Component: centrosome; chromatin; cyclin-dependent protein kinase holoenzyme complex; nuclear body; nuclear lamina; nuclear replication fork; nucleoplasm; nucleus; PCNA complex; PCNA-p21 complex; replication fork; replisome

Molecular Function: chromatin binding; damaged DNA binding; dinucleotide insertion or deletion binding; DNA polymerase binding; enzyme binding; estrogen receptor binding; histone acetyltransferase binding; identical protein binding; MutLalpha complex binding; protein binding; purine-specific mismatch base pair DNA N-glycosylase activity; receptor tyrosine kinase binding

Biological Process: base-excision repair, gap-filling; cellular response to hydrogen peroxide; cellular response to UV; cellular response to xenobiotic stimulus; DNA damage response, detection of DNA damage; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; epithelial cell differentiation; error-free translesion synthesis; error-prone translesion synthesis; estrous cycle; heart development; liver regeneration; mismatch repair; mitotic telomere maintenance via semi-conservative replication; negative regulation of transcription by RNA polymerase II; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 5'-to lesion; positive regulation of deoxyribonuclease activity; positive regulation of DNA repair; positive regulation of DNA replication; protein ubiquitination; regulation of transcription involved in G1/S transition of mitotic cell cycle; replication fork processing; response to cadmium ion; response to dexamethasone; response to estradiol; response to L-glutamate; telomere maintenance; telomere maintenance via semi-conservative replication; transcription-coupled nucleotide-excision repair; translesion synthesis; viral process

Disease: Ataxia-telangiectasia-like Disorder 2

Reference #: P12004 (UniProtKB)

Alt. Names/Synonyms: Cyclin; DNA polymerase delta auxiliary protein; MGC8367; PCNA; Proliferating cell nuclear antigen

Gene Symbols: PCNA

Molecular weight: 28,769 Da

Basal Isoelectric point: 4.57 Predict pI for various phosphorylation states

Protein-Specific Antibodies or siRNAs from Cell Signaling Technology^®

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PCNA

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