PRPF8 Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site. Widely expressed. Note: This description may include information from UniProtKB.
Protein type: RNA splicing; RNA-binding; Spliceosome
Chromosomal Location of Human Ortholog: 11 B5|11 45.92 cM
Cellular Component:  catalytic step 2 spliceosome; nucleus; small nuclear ribonucleoprotein complex; spliceosomal complex; U2-type catalytic step 2 spliceosome; U2-type precatalytic spliceosome; U4/U6 x U5 tri-snRNP complex; U5 snRNP
Molecular Function:  K63-linked polyubiquitin modification-dependent protein binding; pre-mRNA intronic binding; RNA binding; second spliceosomal transesterification activity; U1 snRNA binding; U2 snRNA binding; U5 snRNA binding; U6 snRNA binding
Biological Process:  cellular response to lipopolysaccharide; cellular response to tumor necrosis factor; mRNA processing; mRNA splicing, via spliceosome; RNA splicing; spliceosomal tri-snRNP complex assembly
Reference #:  Q99PV0 (UniProtKB)
Alt. Names/Synonyms: AU019467; D11Bwg0410e; DBF3/P; DBF3/PRP8; OTTMUSP00000006504; pre-mRNA processing factor 8; Pre-mRNA-processing-splicing factor 8; Prp8; Prpf8; Sfprp8l; Splicing factor Prp8; splicing factor PRP8-like
Gene Symbols: Prpf8
Molecular weight: 273,616 Da
Basal Isoelectric point: 8.95  Predict pI for various phosphorylation states
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Protein Structure Not Found.

Cross-references to other databases:  STRING  |  Reactome  |  BioGPS  |  Pfam  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene