iNOS Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Amino Acid Metabolism - arginine and proline; EC; Oxidoreductase
Chromosomal Location of Human Ortholog: 11 B5|11 46.74 cM
Cellular Component:  cortical cytoskeleton; cytoplasm; cytosol; extracellular space; nucleus; perinuclear region of cytoplasm; peroxisome; plasma membrane; vesicle membrane
Molecular Function:  actin binding; arginine binding; beta-catenin binding; cadherin binding; calmodulin binding; cAMP-dependent protein kinase regulator activity; flavin adenine dinucleotide binding; FMN binding; heme binding; Hsp90 protein binding; metal ion binding; NADP binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; nitric-oxide synthase binding; oxidoreductase activity; protein binding; protein homodimerization activity; protein kinase binding; signaling receptor binding; tetrahydrobiopterin binding
Biological Process:  arginine catabolic process; cellular response to cytokine stimulus; cellular response to drug; cellular response to interferon-gamma; cellular response to lipopolysaccharide; cellular response to organic cyclic compound; circadian rhythm; defense response to bacterium; inflammatory response; interleukin-6 secretion; interleukin-8 secretion; negative regulation of blood pressure; negative regulation of gene expression; negative regulation of protein catabolic process; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; oxidation-reduction process; peptidyl-cysteine S-nitrosylation; positive regulation of guanylate cyclase activity; positive regulation of killing of cells of other organism; prostaglandin secretion; regulation of cell proliferation; regulation of cytokine production involved in inflammatory response; regulation of insulin secretion; response to bacterium; response to hormone; response to hypoxia; response to lipopolysaccharide; superoxide metabolic process
Reference #:  P29477 (UniProtKB)
Alt. Names/Synonyms: i-NOS; inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; iNOS; Inosl; MAC-NOS; Macrophage NOS; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; Nitric oxide synthase, inducible; NOS type II; Nos-2; NOS-II; Nos2; Nos2a; OTTMUSP00000000202
Gene Symbols: Nos2
Molecular weight: 130,575 Da
Basal Isoelectric point: 7.76  Predict pI for various phosphorylation states
CST Pathways:  Angiogenesis  |  Insulin Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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Protein Structure Not Found.

Cross-references to other databases:  STRING  |  Reactome  |  BioGPS  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene