Chk2 a CAMK protein kinase of the Rad53 family. A cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. Phosphorylates and inhibits CDC25C, preventing entry into mitosis, p53, leading to cell cycle arrest in G1, and BRCA1, restoring survival after DNA damage. Twelve splice variant isoforms have been described for human Chk2. LOF mutants cause Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype also caused by p53 mutations. Familial mutations also associated with prostate and breast cancer, and mutations also seen in a variety of sporadic cancers and cell lines. Note: This description may include information from UniProtKB.
Protein type: CAMK group; EC; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); RAD53 family; Tumor suppressor
Chromosomal Location of Human Ortholog: 12q16
Cellular Component:  chromosome, telomeric region; cytoplasm; Golgi apparatus; nucleoplasm; nucleus; PML body
Molecular Function:  ATP binding; identical protein binding; kinase activity; protein binding; protein homodimerization activity; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; ubiquitin protein ligase binding
Biological Process:  apoptotic process; cellular protein catabolic process; cellular response to bisphenol A; cellular response to DNA damage stimulus; cellular response to drug; cellular response to gamma radiation; DNA damage checkpoint; DNA damage induced protein phosphorylation; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; double-strand break repair; G2/M transition of mitotic cell cycle; intrinsic apoptotic signaling pathway in response to DNA damage; intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; mitotic DNA damage checkpoint; mitotic spindle assembly; negative regulation of cell cycle arrest; negative regulation of DNA damage checkpoint; peptidyl-serine phosphorylation; positive regulation of anoikis; positive regulation of protein phosphorylation; positive regulation of transcription, DNA-templated; protein autophosphorylation; protein phosphorylation; protein stabilization; regulation of protein catabolic process; regulation of transcription, DNA-templated; replicative cell aging; response to gamma radiation; response to glycoside; signal transduction in response to DNA damage; signal transduction involved in intra-S DNA damage checkpoint
Reference #:  Q9R019 (UniProtKB)
Alt. Names/Synonyms: Checkpoint kinase Chk2; Chek2; Chk2; CHK2 checkpoint homolog (S. pombe); CHK2 checkpoint homolog (S. pombe), isoform CRA_a; protein kinase Chk2; Rad53
Gene Symbols: Chek2
Molecular weight: 60,930 Da
Basal Isoelectric point: 6.06  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  G1/S Checkpoint  |  G2/M DNA Damage Checkpoint
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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Cross-references to other databases:  STRING  |  Reactome  |  BioGPS  |  Pfam  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene