PRKD1 a serine/threonine-protein kinase that converts transient signals initiated by PKC into prolonged physiological effects. Involved in Golgi membrane trafficking, cell survival, cell migration, cell differentiation, and cell proliferation. Plays a role in cardiac hypertrophy, VEGFA-induced angiogenesis, genotoxic-induced apoptosis and flagellin-stimulated inflammatory response. Acts downstream of the heterotrimeric G-protein beta/gamma-subunit complex to maintain the structural integrity of the Golgi membranes, and is required for protein transport along the secretory pathway. In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane. May act by activating the lipid kinase phosphatidylinositol 4-kinase beta (PI4KB) at the TGN for the local synthesis of phosphorylated inositol lipids, which induces a sequential production of DAG, phosphatidic acid (PA) and lyso-PA (LPA) that are necessary for membrane fission and generation of specific transport carriers to the cell surface. Under oxidative stress, is phosphorylated at Tyr-463 via SRC-ABL1 and contributes to cell survival by activating IKK complex and subsequent nuclear translocation and activation of NFKB1. Involved in cell migration by regulating integrin alpha-5/beta-3 recycling and promoting its recruitment in newly forming focal adhesion. In osteoblast differentiation, mediates the bone morphogenetic protein 2 (BMP2)-induced nuclear export of HDAC7, which results in the inhibition of HDAC7 transcriptional repression of RUNX2. In neurons, plays an important role in neuronal polarity by regulating the biogenesis of TGN-derived dendritic vesicles, and is involved in the maintenance of dendritic arborization and Golgi structure in hippocampal cells. May potentiate mitogenesis induced by the neuropeptide bombesin or vasopressin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression. Plays an important role in the proliferative response induced by low calcium in keratinocytes, through sustained activation of MAPK1/3 (ERK1/2) pathway. Downstream of novel PKC signaling, plays a role in cardiac hypertrophy by phosphorylating HDAC5, which in turn triggers XPO1/CRM1-dependent nuclear export of HDAC5, MEF2A transcriptional activation and induction of downstream target genes that promote myocyte hypertrophy and pathological cardiac remodeling. Mediates cardiac troponin I (TNNI3) phosphorylation at the PKA sites, which results in reduced myofilament calcium sensitivity, and accelerated crossbridge cycling kinetics. The PRKD1-HDAC5 pathway is also involved in angiogenesis by mediating VEGFA-induced specific subset of gene expression, cell migration, and tube formation. In response to VEGFA, is necessary and required for HDAC7 phosphorylation which induces HDAC7 nuclear export and endothelial cell proliferation and migration. During apoptosis induced by cytarabine and other genotoxic agents, PRKD1 is cleaved by caspase-3 at Asp-378, resulting in activation of its kinase function and increased sensitivity of cells to the cytotoxic effects of genotoxic agents. In epithelial cells, is required for transducing flagellin-stimulated inflammatory responses by binding and phosphorylating TLR5, which contributes to MAPK14/p38 activation and production of inflammatory cytokines. May play a role in inflammatory response by mediating activation of NF-kappa-B. May be involved in pain transmission by directly modulating TRPV1 receptor. Plays a role in activated KRAS-mediated stabilization of ZNF304 in colorectal cancer (CRC) cells. Regulates nuclear translocation of transcription factor TFEB in macrophages upon live S.enterica infection. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily. Note: This description may include information from UniProtKB.

Protein type: Autophagy; CAMK group; EC 2.7.11.13; Kinase, protein; Motility/polarity/chemotaxis; PKD family; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of rat Ortholog: 6q22

Cellular Component: autophagosome membrane; cell cortex; cell-cell junction; cytoplasm; cytosol; Golgi apparatus; membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; trans-Golgi network; Z disc

Molecular Function: ATP binding; calcium-dependent protein kinase C activity; heat shock protein binding; identical protein binding; kinase activity; metal ion binding; protein kinase activity; protein kinase C activity; protein kinase C binding; protein serine kinase activity; protein serine/threonine kinase activity

Biological Process: angiogenesis; apoptotic process; cell differentiation; cellular response to amino acid starvation; cellular response to angiotensin; cellular response to endothelin; cellular response to hydroperoxide; cellular response to norepinephrine stimulus; cellular response to organic cyclic compound; cellular response to oxidative stress; cellular response to phorbol 13-acetate 12-myristate; cellular response to vascular endothelial growth factor stimulus; defense response to Gram-negative bacterium; Golgi organization; Golgi vesicle transport; inflammatory response; innate immune response; intracellular signal transduction; nervous system development; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of angiogenesis; positive regulation of autophagy; positive regulation of blood vessel endothelial cell migration; positive regulation of cell size; positive regulation of CREB transcription factor activity; positive regulation of endothelial cell chemotaxis; positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway; positive regulation of endothelial cell migration; positive regulation of endothelial cell proliferation; positive regulation of gene expression; positive regulation of histone deacetylase activity; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of neuron projection development; positive regulation of NF-kappaB transcription factor activity; positive regulation of NLRP3 inflammasome complex assembly; positive regulation of osteoblast differentiation; positive regulation of peptide hormone secretion; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein export from nucleus; positive regulation of protein import into nucleus; positive regulation of sarcomere organization; positive regulation of transcription by RNA polymerase II; protein autophosphorylation; protein kinase D signaling; protein phosphorylation; regulation of keratinocyte proliferation; regulation of release of sequestered calcium ion into cytosol; regulation of skeletal muscle contraction by modulation of calcium ion sensitivity of myofibril; response to norepinephrine; vascular endothelial growth factor receptor signaling pathway

Reference #: Q9WTQ1 (UniProtKB)

Alt. Names/Synonyms: KPCD1; nPKC-D1; nPKC-mu; Pkcm; Pkd; Prkcm; Prkd1; Protein kinase C mu type; protein kinase C, mu; Protein kinase D; protein kinase D1; Serine/threonine-protein kinase D1

Gene Symbols: Prkd1

Molecular weight: 102,043 Da

Basal Isoelectric point: 6.08 Predict pI for various phosphorylation states

Protein-Specific Antibodies, siRNAs or Recombinant Proteins from Cell Signaling Technology^®

Select Structure to View Below
PRKD1

Substrate Sequence Logo

Open Substrate Page