NEK2 Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGO1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly. NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis. Involved in the regulation of centrosome disjunction. Isoform 1: Phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2: Not present in the nucleolus and, in contrast to isoform 1, does not phosphorylate and activate NEK11 in G1/S-arrested cells. Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. Isoform 1 and isoform 4 are expressed in the testis. Up-regulated in various cancer cell lines, as well as primary breast tumors. 4 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.

Protein type: EC 2.7.11.1; Kinase, protein; NEK family; NEK2 subfamily; Nucleolus; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of human Ortholog: 1q32.3

Cellular Component: centrosome; condensed nuclear chromosome; cytoplasm; cytosol; kinetochore; microtubule; midbody; nucleolus; nucleoplasm; nucleus; protein-containing complex; spindle pole

Molecular Function: ATP binding; metal ion binding; protein binding; protein kinase activity; protein phosphatase binding; protein serine kinase activity; protein serine/threonine kinase activity

Biological Process: blastocyst development; cell division; centrosome separation; chromosome segregation; meiotic cell cycle; mitotic cell cycle; mitotic spindle assembly; negative regulation of centriole-centriole cohesion; negative regulation of DNA binding; positive regulation of telomerase activity; positive regulation of telomere capping; positive regulation of telomere maintenance via telomerase; protein autophosphorylation; protein phosphorylation; regulation of attachment of spindle microtubules to kinetochore; regulation of mitotic centrosome separation; regulation of mitotic nuclear division; spindle assembly

Disease: Retinitis Pigmentosa 67

Reference #: P51955 (UniProtKB)

Alt. Names/Synonyms: HSPK 21; HsPK21; NEK2; NEK2A; Never in mitosis A-related kinase 2; NIMA (never in mitosis gene a)-related kinase 2; NIMA related kinase 2; NimA-like protein kinase 1; NimA-related protein kinase 2; NLK1; PPP1R111; protein phosphatase 1, regulatory subunit 111; RP67; Serine/threonine-protein kinase Nek2

Gene Symbols: NEK2

Molecular weight: 51,763 Da

Basal Isoelectric point: 9.03 Predict pI for various phosphorylation states

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NEK2

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