PKACA Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Isoform 2 phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation. Phosphorylates HSF1; this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. Isoform 1 is ubiquitous. Isoform 2 is sperm-specific and is enriched in pachytene spermatocytes but is not detected in round spermatids. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.
Protein type: AGC group; EC 2.7.11.11; Kinase, protein; PKA family; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)
Cellular Component:  acrosomal vesicle; cAMP-dependent protein kinase complex; cytoplasm; mitochondrion; neuromuscular junction; nucleus; perinuclear region of cytoplasm; plasma membrane; sperm flagellum
Molecular Function:  ATP binding; cAMP-dependent protein kinase activity; protein binding; protein domain specific binding; protein kinase A regulatory subunit binding; protein kinase activity; protein serine/threonine kinase activity
Biological Process:  cellular response to glucose stimulus; cellular response to heat; mesoderm formation; peptidyl-serine phosphorylation; protein kinase A signaling; protein phosphorylation
Reference #:  P00517 (UniProtKB)
Alt. Names/Synonyms: alpha-catalytic subunit; cAMP-dependent protein kinase; cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase, alpha-catalytic subunit; KAPCA; kinase PKA C-alpha; PKA C-alpha; PKA-alpha; PKACA; PRKACA; protein kinase A
Gene Symbols: PRKACA
Molecular weight: 40,620 Da
Basal Isoelectric point: 8.71  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  AMPK Signaling  |  GPCR Signaling to MAPKs  |  Growth And Differentiation Control by MAPKs  |  Hedgehog Signaling  |  Inhibition of Apoptosis  |  Insulin Receptor Signaling  |  Microtubule Dynamics  |  Mitochondrial Control of Apoptosis  |  NF-kB Signaling  |  Parkinson's Disease
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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PKACA

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Cross-references to other databases:  STRING  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Phospho.ELM  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  Ensembl Protein